Effects of Verapamil, Ouabain, and Ethacrynic Acid on Calcium Retention in Perfused Rat Kidneys

A Sprague‐Dawley rat kidney perfusion technique was used in situ to study the effect of verapamil, ouabain, and ethacrynic acid on renal calcium retention. The technique involves perfusion of the kidneys via the abdominal aorta and then through the left and right renal arteries and dorsal aorta. Ver...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1992-12, Vol.81 (12), p.1178-1180
Main Authors: Bikhazi, Anwar B., Medlej‐Hashim, Myrna, El‐Kasti, Muna
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Calcium - metabolism
Calcium-Binding Proteins - metabolism
Ethacrynic Acid - pharmacology
Female
Fundamental and applied biological sciences. Psychology
Kidney - drug effects
Kidney - metabolism
Kinetics
Ouabain - pharmacology
Perfusion
Rats
Rats, Sprague-Dawley
Sodium - physiology
Verapamil - pharmacology
Vertebrates: urinary system
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Summary:A Sprague‐Dawley rat kidney perfusion technique was used in situ to study the effect of verapamil, ouabain, and ethacrynic acid on renal calcium retention. The technique involves perfusion of the kidneys via the abdominal aorta and then through the left and right renal arteries and dorsal aorta. Verapamil (1mM) in Krebs‐improved Ringer solution increased calcium retention in the kidneys by ~ 117% compared with controls. With Na‐free Krebs‐improved Ringer solution, calcium retention increased by only 92%. However, in Krebs‐improved Ringer solutions containing 59 and 122 mequivalents of Na, calcium retention in the kidney increased by 46 and 43%, respectively, compared with controls. With Krebs‐improved Ringer solution containing 15mM ouabain, calcium retention in the kidney decreased by 29.5%, whereas with 15mM ouabain plus 1mM ethacrynic acid in the perfusate, the effect on calcium retention in the kidney was insignificant compared with controls. These results suggest that two sodium‐dependent calcium‐transporting systems exist at the peritubular side of the kidney tubules: (1) a Na+–Ca+2 countertransport system sensitive to verapamil and (2) a Na+–Ca+2 cotransport system sensitive to the intracellular concentration of sodium.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600811210