A molecular phylogeny of Pythium insidiosum

Sequence analysis of the ribosomal DNA internal transcribed spacers (ITS) was used to establish phylogenetic relationships among 23 isolates of Pythium insidiosum, the etiological agent of pythiosis in mammals. The isolates were divided into three distinct clades that exhibited significant geographi...

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Bibliographic Details
Published in:Mycological research 2003-05, Vol.107 (5), p.537-544
Main Authors: SCHURKO, Andrew M., MENDOZA, Leonel, LÉVESQUE, C. André, DÉSAULNIERS, Nicole L., DE COCK, Arthur W. A. M., KLASSEN, Glen R.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
DNA, Ribosomal Spacer - analysis
Dog Diseases - microbiology
Dogs
etiological agents
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Fungi
Horse Diseases - microbiology
Horses
Humans
internal transcribed spacers
Lagenidium giganteum
mammals
Mycoses - microbiology
Mycoses - veterinary
patients
Phylogeny
Phytopathology. Animal pests. Plant and forest protection
Phytophthora megasperma
Plant cytology, morphology, systematics, chorology and evolution
Pythium - classification
Pythium - genetics
Pythium insidiosum
ribosomal DNA
RNA, Ribosomal - genetics
sequence analysis
Sequence Analysis, DNA
Systematics. Morphology. Development cycle. Physiology
Thallophyta
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Summary:Sequence analysis of the ribosomal DNA internal transcribed spacers (ITS) was used to establish phylogenetic relationships among 23 isolates of Pythium insidiosum, the etiological agent of pythiosis in mammals. The isolates were divided into three distinct clades that exhibited significant geographic isolation. Clade I consisted of isolates from North, Central, and South America, while clade II contained isolates from Asia and Australia. Also present in clade II was an isolate from a patient in the USA, but the origin of the infection may have been in the Middle East. Clade III was comprised of isolates from Thailand and the USA. All 23 P. insidiosum isolates were more closely related to each other than to any other Pythium species in this study. Additionally, all Pythium isolates formed a clade separate from both outgroup species, Phytophthora megasperma and Lagenidium giganteum. The ITS sequence results tend to support the existence of geographic variants or cryptic speciation within P. insidiosum. The sequence information obtained also provides an abundance of data for applications in the diagnosis of pythiosis and identification of P. insidiosum from clinical samples.
ISSN:0953-7562
1469-8102
DOI:10.1017/S0953756203007718