A recombinant anti-erbB2, scFv-Fc-IL-2 fusion protein retains antigen specificity and cytokine function

Elevated erbB2 expression is detected in many in situ and invasive human ductal carcinomas. Anti-erbB2 antibody directed at the extracellular domain of erbB2 can result in an antitumor response in some patients with tumors overexpressing erbB2 oncoprotein. By combining interleukin 2 (IL-2) activitie...

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Bibliographic Details
Published in:Biotechnology letters 2003-05, Vol.25 (10), p.815-819
Main Authors: Shi, Ming, Xie, Zhigang, Feng, Jiannan, Sun, Yingxun, Yu, Ming, Shen, Beifen, Guo, Ning
Format: Article
Language:English
Subjects:
Animals
Antigens
Biological and medical sciences
CHO Cells
Cricetinae
Cytokines - immunology
Fundamental and applied biological sciences. Psychology
Humans
Immunoglobulin Fragments - biosynthesis
Immunoglobulin Fragments - genetics
Immunoglobulin Fragments - immunology
Immunoglobulin Fragments - isolation & purification
Immunoglobulin G - genetics
Immunoglobulin G - metabolism
Immunotherapy
Interleukin-2 - genetics
Interleukin-2 - metabolism
Molecular weight
Pharmacokinetics
Proteins
Receptor, ErbB-2 - immunology
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
Recombinant Fusion Proteins - isolation & purification
Sensitivity and Specificity
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Summary:Elevated erbB2 expression is detected in many in situ and invasive human ductal carcinomas. Anti-erbB2 antibody directed at the extracellular domain of erbB2 can result in an antitumor response in some patients with tumors overexpressing erbB2 oncoprotein. By combining interleukin 2 (IL-2) activities with a tumor specific antibody, immunotherapy of tumors might be more effective in the future. In this study, a fusion protein consisting of erbB2 single chain antibody (scFv), Fc fragment of human IgG1 and IL-2 was constructed. The molecular weight of fusion proteins is 66 kDa, only one third of whole antibody-IL-2 fusion protein or 44% whole Ig molecule. The fusion proteins retained the activities of both antigen binding and IL-2. The scFv-Fc-IL-2 fusion protein may have advantages over whole antibody-IL-2 fusion proteins, such as smaller molecule, better activity of penetration, more favorable pharmacokinetic properties.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1023556523735