Preconditioning with erythropoietin protects against subsequent ischemia‐reperfusion injury in rat kidney

ABSTRACT Improving the ability of the kidney to tolerate ischemic injury has important implications. We investigated the effect of recombinant human erythropoietin (rHuEPO) treatment on subsequent ischemia/reperfusion (I/R) injury and evaluated the role of heat shock protein (HSP) 70 in rHuEPO‐induc...

Full description

Saved in:
Bibliographic Details
Published in:The FASEB journal 2003-09, Vol.17 (12), p.1754-1755
Main Authors: Yang, Chul Woo, Li, Can, Young Jung, Ju, Shin, Seok Joon, Choi, Bum Soon, Lim, Sun Woo, Sun, Bo Kyung, Kim, Yong Soo, Kim, Jin, Chang, Yoon Sik, Bang, Byung Kee
Format: Article
Language:English
Subjects:
Animals
Apoptosis
erythropoietin
Erythropoietin - therapeutic use
heat shock protein 70
HSP70 Heat-Shock Proteins - physiology
Ischemic Preconditioning
Kidney - metabolism
Kidney - pathology
Kidney Diseases - metabolism
Kidney Diseases - pathology
Kidney Diseases - prevention & control
Mitogen-Activated Protein Kinases - metabolism
Models, Biological
Rats
Recombinant Proteins
renal ischemia-reperfusion
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
Reperfusion Injury - prevention & control
Signal Transduction
stress kinase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ABSTRACT Improving the ability of the kidney to tolerate ischemic injury has important implications. We investigated the effect of recombinant human erythropoietin (rHuEPO) treatment on subsequent ischemia/reperfusion (I/R) injury and evaluated the role of heat shock protein (HSP) 70 in rHuEPO‐induced renal protection. rHuEPO (3000 U/kg) was administered 24 h before I/R injury, and rats were killed at 24, 48, and 72 h after I/R injury. Pretreatment of rHuEPO resulted in the following: i) decreased serum creatinine level; ii) decreased tubular cell apoptosis and necrosis, measured by DNA fragmentation analysis and TUNEL staining and histomorphological criteria; iii) decreased tubular cell proliferation as determined by proliferating cell nuclear antigen expression; iv) increased bcl‐2 protein and decreased caspase 3 activity; and v) decreased JNK expression. rHuEPO treatment increased HSP70 expression in a dose‐dependent manner in normal rat kidneys, and inhibition of HSP70 expression by quercetin eliminated the renoprotective effect of rHuEPO in ischemic kidneys. Our study demonstrates that rHuEPO has a protective effect on subsequent I/R injury and that this effect is associated with induction of HSP70. Our study provides a new avenue for therapy to prevent renal damage after I/R injury.
ISSN:0892-6638
1530-6860
DOI:10.1096/fj.02-1191fje