A functional relationship between NuMA and kid is involved in both spindle organization and chromosome alignment in vertebrate cells

We examined spindle morphology and chromosome alignment in vertebrate cells after simultaneous perturbation of the chromokinesin Kid and either NuMA, CENP-E, or HSET. Spindle morphology and chromosome alignment after simultaneous perturbation of Kid and either HSET or CENP-E were no different from w...

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Bibliographic Details
Published in:Molecular biology of the cell 2003-09, Vol.14 (9), p.3541-3552
Main Authors: Levesque, Aime A, Howard, Louisa, Gordon, Michael B, Compton, Duane A
Format: Article
Language:English
Subjects:
Antigens, Nuclear
Cell Cycle Proteins
Cells, Cultured
Chromosomal Proteins, Non-Histone - metabolism
Chromosome Positioning
Chromosomes - metabolism
Chromosomes - ultrastructure
DNA-Binding Proteins - metabolism
Humans
Kinesin - metabolism
Microscopy, Electron
Microscopy, Fluorescence
Mitosis
Nuclear Matrix-Associated Proteins
Nuclear Proteins - metabolism
Spindle Apparatus - metabolism
Spindle Apparatus - ultrastructure
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Summary:We examined spindle morphology and chromosome alignment in vertebrate cells after simultaneous perturbation of the chromokinesin Kid and either NuMA, CENP-E, or HSET. Spindle morphology and chromosome alignment after simultaneous perturbation of Kid and either HSET or CENP-E were no different from when either HSET or CENP-E was perturbed alone. However, short bipolar spindles with organized poles formed after perturbation of both Kid and NuMA in stark contrast to splayed spindle poles observed after perturbation of NuMA alone. Spindles were disorganized if Kid, NuMA, and HSET were perturbed, indicating that HSET is sufficient for spindle organization in the absence of Kid and NuMA function. In addition, chromosomes failed to align efficiently at the spindle equator after simultaneous perturbation of Kid and NuMA despite appropriate kinetochore-microtubule interactions that generated chromosome movement at normal velocities. These data indicate that a functional relationship between the chromokinesin Kid and the spindle pole organizing protein NuMA influences spindle morphology, and we propose that this occurs because NuMA forms functional linkages between kinetochore and nonkinetochore microtubules at spindle poles. In addition, these data show that both Kid and NuMA contribute to chromosome alignment in mammalian cells.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.e03-02-0082