La Crosse virus infection of Aedes triseriatus (Diptera: Culicidae) ovaries before dissemination of virus from the midgut

La Crosse (LAC) virus is efficiently transmitted transovarially by the mosquito Aedes triseriatus (Say). To determine the time course and tropisms of LAC virus infection of ovaries, immunofluorescent antibody staining, in situ hybridization, and reverse transcription-polymerase chain reaction techni...

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Bibliographic Details
Published in:Journal of medical entomology 1998-07, Vol.35 (4), p.567-572
Main Authors: Chandler, L.J. (University of Texas Medical Branch, Galveston, TX.), Blair, C.D, Beaty, B.J
Format: Article
Language:English
Subjects:
Aedes - virology
AEDES TRISERIATUS
Animals
ANTIGENE
ANTIGENOS
ANTIGENS
ARN
Biological and medical sciences
BUNYAVIRIDAE
Cells, Cultured
Digestive System
DISEASE TRANSMISSION
DISEASE VECTORS
DISSEMINATED INFECTIONS
EPIDEMIOLOGIA
EPIDEMIOLOGIE
EPIDEMIOLOGY
Female
Fundamental and applied biological sciences. Psychology
INFECCION
INFECTION
INTESTIN
INTESTINES
INTESTINOS
La Crosse virus - genetics
LOCALIZATION
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
Mice
OVAIRE
OVARIES
OVARIOS
Ovary - virology
PATHOGENESE
PATHOGENESIS
PATOGENESIS
Polymerase Chain Reaction
RNA
RNA, Viral
TRANSMISION DE ENFERMEDADES
TRANSMISSION DES MALADIES
TRANSOVARIAL TRANSMISSION
TROPISME
TROPISMO
TROPISMS
VECTEUR DE MALADIE
VECTORES
VECTORS
Vectors. Intermediate hosts
VIRAL ANTIGENS
VIRAL TROPISM
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Description
Summary:La Crosse (LAC) virus is efficiently transmitted transovarially by the mosquito Aedes triseriatus (Say). To determine the time course and tropisms of LAC virus infection of ovaries, immunofluorescent antibody staining, in situ hybridization, and reverse transcription-polymerase chain reaction techniques were used to detect viral antigen and RNA in the ovaries. LAC virus was detected in the ovaries (presumably in calyx tissues) by all 3 assays at day 2 after infection and before dissemination from the midgut on day 6. Apparently, ovaries can become infected by mechanisms other than by dissemination of virus from a midgut infection. By days 8-14 after infection, virus analytes became detectable in many tissues within the ovary including follicular epithelium, oocytes, nurse cells, and calyx, reflecting the remarkable host parasite relationship between LAC virus and its mosquito vector
ISSN:0022-2585
1938-2928
DOI:10.1093/jmedent/35.4.567