NOR lateral asymmetry and its effect on satellite association in BrdU-labeled human lymphocyte cultures

Second generation BrdU-labeled acrocentric chromosomes exhibit NOR lateral asymmetry (NLA) in metaphases that have been sequentially stained with silver and the Hoechst-Giemsa sister chromatid differential (SCD) technique. The NLA presumably results from suppression of NOR activity in the doubly-sub...

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Bibliographic Details
Published in:Human genetics 1981-01, Vol.59 (3), p.259-262
Main Authors: Strobel, R J, Pathak, S, Hsu, T C
Format: Article
Language:English
Subjects:
Adult
Azure Stains
Bromodeoxyuridine - pharmacology
Cells, Cultured
Chromosome Banding
DNA, Satellite
Humans
Lymphocytes - ultrastructure
Male
Metaphase
Nucleic Acid Conformation
Nucleolus Organizer Region - ultrastructure
Silver
Staining and Labeling
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Summary:Second generation BrdU-labeled acrocentric chromosomes exhibit NOR lateral asymmetry (NLA) in metaphases that have been sequentially stained with silver and the Hoechst-Giemsa sister chromatid differential (SCD) technique. The NLA presumably results from suppression of NOR activity in the doubly-substituted chromatid. Examination of single chromatid (NOR) associations in pairs of acrocentrics reveals that light chromatids associate less frequently than dark chromatids and that the frequency distribution of dark and light alignment configurations can be explained by this differential tendency to associate. Thus, it appears that a hypothesis of non-random chromatid segregation as an explanation for non-random chromatid alignments in associating acrocentric chromosomes is unwarranted.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF00283676