The use of viable hepatocytes to study the hormonal control of glycogenolysis in the chicken

The rapid isolation of high yields of parenchymal cells from chicken liver is described. Stringent tests of viability show that the isolated hepatocytes are both structurally and metabolically similar to those in intact liver. During incubation viability decreased and the significance of this change...

Full description

Saved in:
Bibliographic Details
Published in:Molecular and cellular biochemistry 1978-04, Vol.19 (2), p.81-92
Main Authors: Dickson, A J, Anderson, C E, Langslow, D R
Format: Article
Language:English
Subjects:
Adenosine Diphosphate - metabolism
Adenosine Triphosphate - metabolism
Animals
Bucladesine - pharmacology
Cell Separation - methods
Cell Survival
Chickens
Epinephrine - pharmacology
Fasting
Glucagon - pharmacology
Glucose - biosynthesis
In Vitro Techniques
Lactates - pharmacology
Liver - cytology
Liver - metabolism
Liver Glycogen - metabolism
Oxygen Consumption
Pyruvates - pharmacology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The rapid isolation of high yields of parenchymal cells from chicken liver is described. Stringent tests of viability show that the isolated hepatocytes are both structurally and metabolically similar to those in intact liver. During incubation viability decreased and the significance of this change on the interpretation of metabolic experiments is discussed. Lactate was a much more effective gluconeogenic precursor than pyruvate even in the presence of additional reducing equivalents. Hepatocytes isolated from fed chickens produced glucose from glycogen degradation. Glycogenolysis was stimulated by glucagon, dibutyryl cyclic AMP and adrenaline. Half maximal glucagon effects were elicited by physiological concentrations of the hormone. Glucagon and dibutyryl cyclic AMP stimulated glucagon, dibutyryl cyclic AMP and adrenaline their action was not additive to that of adrenaline.
ISSN:0300-8177
1573-4919
DOI:10.1007/BF00232598