Sustained hyperglycemia in vitro down-regulates the GLUT1 glucose transport system of cultured human term placental trophoblast: a mechanism to protect fetal development?

The trophoblast of human placenta is directly exposed to the maternal circulation. It forms the main barrier to maternal-fetal glucose transport. The present study investigated the effect of sustained hyperglycemia in vitro on the glucose transport system of these cells. Trophoblasts isolated from t...

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Bibliographic Details
Published in:The FASEB journal 1998-09, Vol.12 (12), p.1221-1231
Main Authors: Hahn, T, Barth, S, Weiss, U, Mosgoeller, W, Desoye, G
Format: Article
Language:English
Subjects:
3-O-Methylglucose - metabolism
Biological Transport
Cell Survival
Cells, Cultured
Embryonic and Fetal Development - physiology
Female
Glucose - metabolism
Glucose - pharmacology
Glucose - physiology
Glucose Transporter Type 1
Humans
Hyperglycemia
Kinetics
Microvilli - ultrastructure
Monosaccharide Transport Proteins - biosynthesis
Monosaccharide Transport Proteins - genetics
Pregnancy
RNA, Messenger - biosynthesis
Time Factors
Transcription, Genetic
Trophoblasts - cytology
Trophoblasts - physiology
Trophoblasts - ultrastructure
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Summary:The trophoblast of human placenta is directly exposed to the maternal circulation. It forms the main barrier to maternal-fetal glucose transport. The present study investigated the effect of sustained hyperglycemia in vitro on the glucose transport system of these cells. Trophoblasts isolated from term placentas and immunopurified were cultured for 24, 48, and 96 h in DMEM containing either 5.5 (normoglycemia) or 25 mmol/l D-glucose (hyperglycemia), respectively. Initial uptake of glucose was measured using 3-O-[14C]methyl-D-glucose. Kinetic parameters were calculated as K(M) = 73 mmol/l and Vmax = 29 fmol s(-1) per trophoblast cell. Uptake rates of cells cultured under hyperglycemic conditions did not differ at exogenous D-glucose concentrations in the physiological range (1, 5.5, 10, and 15 mmol/l), but were significantly decreased by 25% (P
ISSN:0892-6638
DOI:10.1096/fasebj.12.12.1221