Complete amino acid sequence of bovine beta 2-microglobulin

beta 2-Microglobulin has been isolated and crystallized from bovine colostrum and represents the only crystalline form of this protein reported. The complete sequence of bovine beta 2-microglobulin was determined using only one proteolytic enzyme, Staphylococcus aureus V8 protease. Automated degrada...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1982-03, Vol.257 (5), p.2619-2626
Main Authors: Groves, M L, Greenberg, R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
beta 2-Microglobulin - isolation & purification
Beta-Globulins - isolation & purification
Cattle
Colostrum - analysis
Crystallization
Female
Guinea Pigs
Humans
Mice
Peptide Fragments - analysis
Pregnancy
Rabbits
Species Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:beta 2-Microglobulin has been isolated and crystallized from bovine colostrum and represents the only crystalline form of this protein reported. The complete sequence of bovine beta 2-microglobulin was determined using only one proteolytic enzyme, Staphylococcus aureus V8 protease. Automated degradation of the intact molecule and two large peptides produced by enzymic digestion provided unequivocal placement of all residues. Bovine beta 2-microglobulin, molecular weight 11,630, contains 98 residues as compared with 99 for the human, rabbit, guinea pig, and murine proteins. The valine residue at position 49 in all the aforementioned species is deleted in the bovine variant. This crystalline protein is also uniquely characterized by three di-prolyl sequences in the first third of the molecule. beta 2-Microglobulin is a highly conserved protein, and in a comparison of absolute amino acid sequence differences among species, bovine beta 2- microglobulin ranges from a low of 24 substitutions as compared with the rabbit homologue and 26 for human to 32 for mouse and guinea pig.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)34969-X