Determination of Ethinyl Estradiol in Human Urine by Radiochemical GLC

A radiochemical GLC analysis was developed for 3H-labeled ethinyl estradiol in human urine. The technique was applied to the unconjugated and aglycone fractions of urine collected from women who were dosed orally with: (a) single capsules containing 2.0mg of 3H-quinestrol (900μCi) and 2.5mg of unlab...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1978-08, Vol.67 (8), p.1138-1141
Main Authors: Zuleski, Francis R., Loh, Arthur, Di Carlo, Frederick J.
Format: Article
Language:English
Subjects:
Adult
Chromatography, Gas
Estrogens-ethinyl estradiol
Estrogens—ethinyl estradiol, radiochemical GLC analysis in human urine
Ethinyl Estradiol - urine
ethinyl estradiol in human urine
Ethinyl estradiol-radiochemical GLC analysis in human urine
Female
GLC
GLC, radiochemical—analysis, ethinyl estradiol in human urine
Humans
Methods
Middle Aged
Quinestrol - urine
radiochemical GLC analysis in human urine
Radiochemical GLC-analysis
Radiochemical GLC—analysis, ethinyl estradiol in human urine
radiochemical-analysis
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Summary:A radiochemical GLC analysis was developed for 3H-labeled ethinyl estradiol in human urine. The technique was applied to the unconjugated and aglycone fractions of urine collected from women who were dosed orally with: (a) single capsules containing 2.0mg of 3H-quinestrol (900μCi) and 2.5mg of unlabeled quingestanol acetate dissolved in sesame oil and (6) single tablets containing 100μg of 3H-quinestrol (86μCi). Unconjugated ethinyl estradiol in Day 1 urine collections represented means of 0.02% of the high quinestrol dose and 0.12% of the low dose. Ethinyl estradiol glucuronide in the same collections represented means of 0.55% of the high drug dose and 1.35% of the low dose. The method could detect 1-ng quantities of 3H-ethinyl estradiol and 3H-quinestrol.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600670829