Substrate inhibition of the mitochondrial and cytoplasmic malate dehydrogenases [isoenzymes obtained from pig heart]

• Published in: The Journal of biological chemistry 1978-12, Vol.253 (24), p.8697-8701
• Main Authors: Bernstein, L H, Grisham, M B, Cole, K D, Everse, J
• Format: Article
• Language: English
• Subjects: Animals; Cytoplasm - enzymology; Kinetics; Malate Dehydrogenase - antagonists & inhibitors; Mitochondria, Heart - enzymology; Myocardium - enzymology; NAD - pharmacology; Oxaloacetates - pharmacology; Structure-Activity Relationship; Swine
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(17)34233-3

The mechanism that leads to an inhibition of enzyme activity in the presence of high concentrations of substrate was investigated with the two malate dehydrogenase isoenzymes obtained from pig heart. The inhibition is promoted by an abortive binary complex formed by the enzymes and the enol form of of oxalacelate. Neither the oxidized coenzyme nor the reduced coenzyme appears to be involved in the formation of this complex. These results suggest that the mechanism of substrate inhibition that occurs with the pig heart malate dehydrogenases is different from that observed with the lactate dehydrogenases from chicken hearts. The inhibition constants for oxalacetate are 2.0 mM with the mitochondrial enzyme and 4.5 mM with the cytoplasmic enzyme. Since the in vivo concentration of oxalacetate is reported to be about 10 micrometer, these data suggest that the substrate inhibition that is exhibited by the malate dehydrogenases may not be of any significance in vivo.