Direct Allelic Variation Scanning of the Yeast Genome

As more genomes are sequenced, the identification and characterization of the causes of heritable variation within a species will be increasingly important. It is demonstrated that allelic variation in any two isolates of a species can be scanned, mapped, and scored directly and efficiently without...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1998-08, Vol.281 (5380), p.1194-1197
Main Authors: Winzeler, Elizabeth A., Richards, Dan R., Conway, Andrew R., Goldstein, Alan L., Kalman, Sue, McCullough, Michael J., McCusker, John H., Stevens, David A., Wodicka, Lisa, Lockhart, David J., Davis, Ronald W.
Format: Article
Language:English
Subjects:
Alleles
Biochemistry
Biological and medical sciences
Chromosome mapping
Chromosome Mapping - methods
Chromosomes
Classical genetics, quantitative genetics, hybrids
Coding
Cycloheximide - pharmacology
Deoxyribonucleic acid
DNA
DNA probes
Drug Resistance, Microbial - genetics
Drug Resistance, Multiple - genetics
Fundamental and applied biological sciences. Psychology
Gene Deletion
Genes, Fungal
Genetic aspects
Genetic inheritance
Genetic Linkage
Genetic loci
Genetic Markers
Genetic Techniques
Genetic Variation
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Genome, Fungal
Genomes
Genomics
Genotype
Nucleic Acid Hybridization
Oligonucleotide probes
Phenotype
Recombination, Genetic
Saccharomyces cerevisiae - genetics
Thallophyta, bryophyta
Vegetals
Yeast
Yeast (Food product)
Yeasts
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Summary:As more genomes are sequenced, the identification and characterization of the causes of heritable variation within a species will be increasingly important. It is demonstrated that allelic variation in any two isolates of a species can be scanned, mapped, and scored directly and efficiently without allele-specific polymerase chain reaction, without creating new strains or constructs, and without knowing the specific nature of the variation. A total of 3714 biallelic markers, spaced about every 3.5 kilobases, were identified by analyzing the patterns obtained when total genomic DNA from two different strains of yeast was hybridized to high-density oligonucleotide arrays. The markers were then used to simultaneously map a multidrug-resistance locus and four other loci with high resolution (11 to 64 kilobases).
ISSN:0036-8075
1095-9203
DOI:10.1126/science.281.5380.1194