Distinct Cohesin Complexes Organize Meiotic Chromosome Domains

Meiotic cohesin complexes at centromeres behave differently from those along chromosome arms, but the basis for these differences has remained elusive. The fission yeast cohesin molecule Rec8 largely replaces its mitotic counterpart, Rad21/Scc1, along the entire chromosome during meiosis. Here we sh...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2003-05, Vol.300 (5622), p.1152-1155
Main Authors: Kitajima, Tomoya S., Yokobayashi, Shihori, Yamamoto, Masayuki, Watanabe, Yoshinori
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Division
Cell separation
Centromere - physiology
Centromeres
Chemical properties
Chromatids
Chromatids - physiology
Chromatin
Chromosome Segregation
Chromosomes
Chromosomes, Fungal - physiology
Composition
Fundamental and applied biological sciences. Psychology
Genes
Green Fluorescent Proteins
Heterochromatin
Histones
Luminescent Proteins
Meiosis
Meiosis - physiology
Mitosis
Molecular and cellular biology
Molecular biology
Observations
Phosphoproteins - physiology
Prophase
Schizosaccharomyces
Schizosaccharomyces pombe Proteins - genetics
Schizosaccharomyces pombe Proteins - physiology
Yeasts
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Summary:Meiotic cohesin complexes at centromeres behave differently from those along chromosome arms, but the basis for these differences has remained elusive. The fission yeast cohesin molecule Rec8 largely replaces its mitotic counterpart, Rad21/Scc1, along the entire chromosome during meiosis. Here we show that Rec8 complexes along chromosome arms contain Rec11, whereas those in the vicinity of centromeres have a different partner subunit, Psc3. The arm-associated Rec8-Rec11 complexes are critical for meiotic recombination. The Rec8-Psc3 complexes comprise two different types of assemblies. First, pericentromeric Rec8-Psc3 complexes depend on histone methylation-directed heterochromatin for their localization and are required for cohesion during meiosis II. Second, central core Rec8-Psc3 complexes form independently of heterochromatin and are presumably required for establishing monopolar attachment at meiosis I. These findings define distinct modes of assembly and functions for cohesin complexes at different regions along chromosomes.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1083634