Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension

• Published in: Nature 2003-10, Vol.425 (6960), p.821-824
• Main Authors: Webb, Watt W, Baumgart, Tobias, Hess, Samuel T
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Cell Membrane - chemistry; Cell Membrane - metabolism; Cell membranes. Ionic channels. Membrane pores; Cell structures and functions; Fluids; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; Humanities and Social Sciences; letter; Lipid Bilayers - chemistry; Lipid Bilayers - metabolism; Liposomes - chemistry; Liposomes - metabolism; Membranes; Microscopy, Fluorescence; Models, Biological; Models, Chemical; Molecular and cellular biology; multidisciplinary; Phosphatidylcholines - metabolism; Phosphatidylethanolamines - metabolism; Photons; Science; Science (multidisciplinary); Temperature; Tension
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/nature02013

Lipid bilayer membranes-ubiquitous in biological systems and closely associated with cell function-exhibit rich shape-transition behaviour, including bud formation and vesicle fission. Membranes formed from multiple lipid components can laterally separate into coexisting liquid phases, or domains, with distinct compositions. This process, which may resemble raft formation in cell membranes, has been directly observed in giant unilamellar vesicles. Detailed theoretical frameworks link the elasticity of domains and their boundary properties to the shape adopted by membranes and the formation of particular domain patterns, but it has been difficult to experimentally probe and validate these theories. Here we show that high-resolution fluorescence imaging using two dyes preferentially labelling different fluid phases directly provides a correlation between domain composition and local membrane curvature. Using freely suspended membranes of giant unilamellar vesicles, we are able to optically resolve curvature and line tension interactions of circular, stripe and ring domains. We observe long-range domain ordering in the form of locally parallel stripes and hexagonal arrays of circular domains, curvature-dependent domain sorting, and membrane fission into separate vesicles at domain boundaries. By analysing our observations using available membrane theory, we are able to provide experimental estimates of boundary tension between fluid bilayer domains.