Modulation of ATP-Dependent Chromatin-Remodeling Complexes by Inositol Polyphosphates

Eukaryotes use adenosine triphosphate (ATP)-dependent chromatin-remodeling complexes to regulate gene expression. Here, we show that inositol polyphosphates can modulate the activities of several chromatin-remodeling complexes in vitro. Inositol hexakisphosphate (IP6) inhibits nucleosome mobilizatio...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2003-01, Vol.299 (5603), p.112-114
Main Authors: Shen, Xuetong, Xiao, Hua, Ranallo, Ryan, Wu, Wei-Hua, Wu, Carl
Format: Article
Language:English
Subjects:
Adenosine triphosphatases
Adenosine Triphosphatases - metabolism
Adenosine triphosphate
Adenosine Triphosphate - metabolism
ATP (Adenosine triphosphate)
Biochemistry
Biological and medical sciences
Chromatin
Chromatin - metabolism
DNA
DNA-Binding Proteins - metabolism
Enzymes
Eukaryotes
Fundamental and applied biological sciences. Psychology
Gene expression
Gene expression regulation
Gene Expression Regulation, Fungal
Genes
Hydrolysis
Inositol phosphates
Inositol Phosphates - metabolism
Inositols
Molecular and cellular biology
Molecular genetics
Mutation
Myo-Inositol-1-Phosphate Synthase - genetics
Myo-Inositol-1-Phosphate Synthase - metabolism
Nuclear Proteins
Nucleosomes
Nucleosomes - metabolism
Phytic Acid - metabolism
Phytic Acid - pharmacology
Polyphosphates
Recombinant Proteins - metabolism
Renovations
RNA, Messenger - genetics
RNA, Messenger - metabolism
Signal Transduction
Transcription Factors - metabolism
Yeasts
Yeasts - genetics
Yeasts - metabolism
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Summary:Eukaryotes use adenosine triphosphate (ATP)-dependent chromatin-remodeling complexes to regulate gene expression. Here, we show that inositol polyphosphates can modulate the activities of several chromatin-remodeling complexes in vitro. Inositol hexakisphosphate (IP6) inhibits nucleosome mobilization by NURF, ISW2, and INO80 complexes. In contrast, nucleosome mobilization by the yeast SWI/SNF complex is stimulated by inositol tetrakis-phosphate (IP4) and inositol pentakisphosphate (IP5). We demonstrate that mutations in genes encoding inositol polyphosphate kinases that produce IP4, IP5, and IP6impair transcription in vivo. These results provide a link between inositol polyphosphates, chromatin remodeling, and gene expression.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1078068