Replication Dynamics of the Yeast Genome

Oligonucleotide microarrays were used to map the detailed topography of chromosome replication in the budding yeast Saccharomyces cerevisiae. The times of replication of thousands of sites across the genome were determined by hybridizing replicated and unreplicated DNAs, isolated at different times...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2001-10, Vol.294 (5540), p.115-121
Main Authors: Raghuraman, M. K., Winzeler, Elizabeth A., Collingwood, David, Hunt, Sonia, Wodicka, Lisa, Conway, Andrew, Lockhart, David J., Davis, Ronald W., Brewer, Bonita J., Fangman, Walton L.
Format: Article
Language:English
Subjects:
Algorithms
Base Sequence
Biological and medical sciences
Brewer's yeast
Cells
Cells (Biology)
Cellular biology
Centromere - metabolism
Centromeres
Chromosome mapping
Chromosome replication
Chromosomes
Chromosomes, Fungal - genetics
Chromosomes, Fungal - metabolism
Coordinate systems
DNA microarrays
DNA Replication
DNA, Fungal - biosynthesis
DNA, Fungal - genetics
DNA, Fungal - metabolism
DNA, Intergenic
Eukaryotes
Eukaryotic cells
Fourier Analysis
Fundamental and applied biological sciences. Psychology
Genes
Genes. Genome
Genetics
Genome, Fungal
Genomes
Genomics
Interphase
Kinetics
Mammals
Molecular and cellular biology
Molecular genetics
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis
Profiles
Replication Origin
S Phase
Saccharomyces cerevisiae
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Telomere - metabolism
Telomeres
Topography
Transcription, Genetic
Yeast
Yeasts
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Summary:Oligonucleotide microarrays were used to map the detailed topography of chromosome replication in the budding yeast Saccharomyces cerevisiae. The times of replication of thousands of sites across the genome were determined by hybridizing replicated and unreplicated DNAs, isolated at different times in S phase, to the microarrays. Origin activations take place continuously throughout S phase but with most firings near mid-S phase. Rates of replication fork movement vary greatly from region to region in the genome. The two ends of each of the 16 chromosomes are highly correlated in their times of replication. This microarray approach is readily applicable to other organisms, including humans.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.294.5540.115