Use of laboratory toxicity tests with bivalve and echinoderm embryos to evaluate the bioavailability of copper in San Diego Bay, California, USA

• Published in: Environmental toxicology and chemistry 2005-02, Vol.24 (2), p.415-422
• Main Authors: Rosen, Gunther, Rivera-Duarte, Ignacio, Kear-Padilla, Lora, Chadwick, D. Bart
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Animals; Applied ecology; Bioavailability; Biological and medical sciences; Biological Availability; Bivalvia; California; Copper; Copper - analysis; Copper - pharmacokinetics; Copper - toxicity; Dissolved organic carbon; Echinodermata; Echinodermata - embryology; Echinodermata - metabolism; Ecotoxicology, biological effects of pollution; Embryo-larval development; Embryos; Environmental Monitoring; Environmental protection; Estuaries; Fundamental and applied biological sciences. Psychology; General aspects; Larvae; Larval development; Marine; Mollusks; Seawater - analysis; Seawater - chemistry; Surface water; Total suspended solids; Toxicity; Toxicity Tests; Water analysis; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - pharmacokinetics; Water Pollutants, Chemical - toxicity; Water quality; Water sampling; Water-effect ratio
• ISSN: 0730-7268; 1552-8618
• DOI: 10.1897/04-079R1.1

Copper concentrations in parts of San Diego Bay (CA, USA) exceed ambient water quality criteria (WQC; currently 3.1 μg/L dissolved, U.S. Environmental Protection Agency [U.S. EPA]). In order to better understand the bioavailability of copper to water‐column organisms in the bay, toxicity tests were performed with copper added to surface water collected from various sites in the estuary over a three‐year period. The species and endpoints used, bivalve and echinoderm embryo‐larval development, are among the most sensitive in the U.S. EPA's national toxicity dataset, which is used to derive WQC. No toxicity was observed in ambient bay water samples, as indicated by high proportions of normally developed larvae in control treatments, averaging 93 ± 5% across all sites and all sampling events. Median effects concentrations (EC50), obtained by copper spiking of ambient water samples, ranged from 1.7 to 3.4 times lower at sites located near the mouth compared to sites near the back of the bay. These data indicate a gradient in complexation capacity increasing from the mouth to the back of the bay, which is consistent with similar trends in dissolved organic carbon and total suspended solids. For the bay as a whole, estimates for total recoverable and dissolved water‐effect ratios (WER) ranged from 2.07 to 2.27 and 1.54 to 1.67, respectively. Water‐effect ratios of this magnitude suggest that adoption of a somewhat higher site‐specific WQC for San Diego Bay still would achieve the level of protection that is intended by the WQC guidelines.