Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species

To obtain a restoring and protective calcite layer on degraded limestone, five different strains of the Bacillus sphaericus group and one strain of Bacillus lentus were tested for their ureolytic driven calcium carbonate precipitation. Although all the Bacillus strains were capable of depositing cal...

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Bibliographic Details
Published in:Biodegradation (Dordrecht) 2006-08, Vol.17 (4), p.357-367
Main Authors: Dick, J, Windt, W. de, Graef, B. de, Saveyn, H, Meeren, P. van der, Belie, N. de, Verstraete, W
Format: Article
Language:English
Subjects:
Absorption
Bacillus
Bacillus - chemistry
Bacillus - metabolism
Bacillus lentus
Bacillus sphaericus
Bacteria
biodegradation
Biodegradation of pollutants
Biodegradation, Environmental
biofilm
Biofilms
Biological and medical sciences
bioremediation
Biotechnology
Calcite
Calcium - chemistry
Calcium carbonate
Calcium Carbonate - chemistry
Environment and pollution
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
in vitro culture
Industrial applications and implications. Economical aspects
Limestone
Microbiology
Microscopy, Electron, Scanning
mineralization
Models, Chemical
pollution
Polymers - chemistry
Time Factors
Urea
Urea - chemistry
ureolytic calcium precipitation
Water - chemistry
Zeta potential
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Summary:To obtain a restoring and protective calcite layer on degraded limestone, five different strains of the Bacillus sphaericus group and one strain of Bacillus lentus were tested for their ureolytic driven calcium carbonate precipitation. Although all the Bacillus strains were capable of depositing calcium carbonate, differences occurred in the amount of precipitated calcium carbonate on agar plate colonies. Seven parameters involved in the process were examined: calcite deposition on limestone cubes, pH increase, urea degrading capacity, extracellular polymeric substances (EPS)-production, biofilm formation, zeta-potential and deposition of dense crystal layers. The strain selection for optimal deposition of a dense CaCO(3) layer on limestone, was based on decrease in water absorption rate by treated limestone. Not all of the bacterial strains were effective in the restoration of deteriorated Euville limestone. The best calcite precipitating strains were characterised by high ureolytic efficiency, homogeneous calcite deposition on limestone cubes and a very negative zeta-potential.
ISSN:0923-9820
1572-9729
DOI:10.1007/s10532-005-9006-x