Thrombin and epidermal growth factor become linked to cell surface receptors during mitogenic stimulation

THERE is much evidence that interaction with specific cell surface receptors is a key event in the action of polypeptide hormones and growth factors. Many analyses of hormone–receptor interactions have been carried out based on the assumption that hormone binding to the cell receptors is a reversibl...

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Bibliographic Details
Published in:Nature (London) 1979-04, Vol.278 (5706), p.743-745
Main Authors: BAKER, JOFFRE B, SIMMER, ROBERT L, GLENN, KEVIN C, CUNNINGHAM, DENNIS D
Format: Article
Language:English
Subjects:
Biological Transport
Cell Division - drug effects
Cells, Cultured
Epidermal Growth Factor - metabolism
Epidermal Growth Factor - pharmacology
Humanities and Social Sciences
Humans
Kinetics
letter
Mitogens
multidisciplinary
Peptides - metabolism
Protein Binding
Receptors, Cell Surface - metabolism
Science
Science (multidisciplinary)
Thrombin - metabolism
Thrombin - pharmacology
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Summary:THERE is much evidence that interaction with specific cell surface receptors is a key event in the action of polypeptide hormones and growth factors. Many analyses of hormone–receptor interactions have been carried out based on the assumption that hormone binding to the cell receptors is a reversible reaction 1–3 . Indeed, many studies have demonstrated that bound hormones dissociate from their receptors. However, in most systems the possibility could not be excluded that a significant fraction of the hormone molecules that bind to cells become linked to their receptors. Our present studies with the polypeptide mitogens 125 I-thrombin and 125 I-epidermal growth factor ( 125 I-EGF) show that a significant percentage of the molecules that are specifically bound on human fibroblasts become linked to cell surface receptors. This finding has broad implications for the way in which these molecules might stimulate cell division. It also raises the question of whether linkage of polypeptide hormones to their receptors is a general phenomenon.
ISSN:0028-0836
1476-4687
DOI:10.1038/278743a0