Effect of products derived from hydrolysis of wheat and flaxseed non starch polysaccharides by carbohydrase enzymes on net absorption in enterotoxigenic Escherichia coli (K88) challenged piglet jejunal segments

• Published in: Animal science journal 2010-02, Vol.81 (1), p.63-71
• Main Authors: Kiarie, E.G., University of Manitoba, Winnipeg (Canada), Slominski, B.A, Nyachoti, C.M
• Format: Article
• Language: English
• Subjects: Animal Feed; Animals; Bacterial infections; BLE; Body Fluids - metabolism; Carbohydrates; DIARREA; Diarrhea; Diarrhea - metabolism; Diarrhea - veterinary; DIARRHEE; DIARRHOEA; Diet; E coli; Enterotoxigenic Escherichia coli; Enzymes; ESCHERICHIA COLI; Escherichia coli Infections - metabolism; Escherichia coli Infections - veterinary; Feed science; Flax - chemistry; Flax - metabolism; flaxseed; Glycoside Hydrolases - chemistry; GRAINE DE LIN; HIDROLISIS; Hogs; http://www.fao.org/aos/agrovoc#c_10363; http://www.fao.org/aos/agrovoc#c_12192; http://www.fao.org/aos/agrovoc#c_24940; http://www.fao.org/aos/agrovoc#c_33700; http://www.fao.org/aos/agrovoc#c_3879; http://www.fao.org/aos/agrovoc#c_3924; http://www.fao.org/aos/agrovoc#c_5872; http://www.fao.org/aos/agrovoc#c_6097; http://www.fao.org/aos/agrovoc#c_6211; http://www.fao.org/aos/agrovoc#c_8373; HYDROLYSE; HYDROLYSIS; Infection; INOCULACION; INOCULATION; INTESTIN; INTESTINES; INTESTINOS; Jejunum - drug effects; Jejunum - metabolism; LECHON; LINAZA; LINSEED; non starch polysaccharides hydrolysis products; Perfusion; piglet diarrhea; PIGLETS; POLISACARIDOS; POLYHOLOSIDE; POLYSACCHARIDES; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; PORCELET; PRODUCTOS; PRODUCTS; PRODUIT; Small intestine; Solutes; Starch; Sus scrofa; Swine Diseases - metabolism; TRIGO; Triticum - chemistry; Triticum - metabolism; Triticum aestivum; Water-Electrolyte Balance - drug effects; wheat; WHEATS
• ISSN: 1344-3941; 1740-0929
• DOI: 10.1111/j.1740-0929.2009.00716.x

ABSTRACT Enterotoxigenic Escherichia coli (ETEC) infection results in fluid and electrolyte losses in the small intestine. We investigated the effect of non‐starch polysaccharides (NSP) hydrolysis products of wheat middlings (WM) and flaxseed (FS) on net absorption of fluid and solutes during ETEC challenge. Products were generated by incubating WM and FS with a blend of carbohydrase enzymes to produce 2 products: 80% ethanol‐soluble (ES) and 80% ethanol‐insoluble (EI) which were studied in 2 experiments in which 2 factors were investigated: products (EI vs. ES) and time of ETEC challenge (before vs. after perfusion). Pairs of small‐intestine segments, one non‐challenged and the other ETEC‐challenged were perfused with products for 7.5 h. ETEC reduced fluid absorption by more than 380 µL/cm2 in saline (control) perfused segments, whereas this reduction was significantly (P  0.05) between product and time of challenge was not observed. For WM, products effects on ETEC‐challenged segments were such that perfusion of ES resulted in higher total solute (measured as osmolality) absorption than EI (138 vs. 103 µOsmol/cm2). In conclusion, hydrolysis products from WM and FS were beneficial in maintaining fluid balance during ETEC challenge, suggesting potential in controlling ETEC induced diarrhea in piglets.