Clinical Pharmacokinetics of Tacrolimus after the First Oral Administration in Renal Transplant Recipients on Triple Immunosuppressive Therapy

• Published in: Basic & clinical pharmacology & toxicology 2010-06, Vol.106 (6), p.505-510
• Main Authors: Velickovic‐Radovanovic, Radmila M., Paunovic, Goran, Mikov, Momir, Djordjevic, Vidojko, Stojanovic, Mariola, Catic‐Djordjevic, Aleksandra, Cvetkovic, Tatjana
• Format: Article
• Language: English
• Subjects: Administration, Oral; Adult; Antibodies, Monoclonal - therapeutic use; Area Under Curve; Biological and medical sciences; Blood levels; Drug Monitoring - methods; Enzyme immunoassay; Female; Humans; Immunosuppressive agents; Immunosuppressive Agents - pharmacokinetics; Immunosuppressive Agents - therapeutic use; Kidney Transplantation; Male; Mathematical models; Medical sciences; microparticles; Middle Aged; Oral administration; Pharmacokinetics; Pharmacology. Drug treatments; Recombinant Fusion Proteins - therapeutic use; Regression Analysis; Sampling; Serbia; Tacrolimus; Tacrolimus - pharmacokinetics; Tacrolimus - therapeutic use; Time Factors
• ISSN: 1742-7835; 1742-7843
• DOI: 10.1111/j.1742-7843.2009.00535.x

:  Monitoring of tacrolimus blood concentration is of utmost importance in the management of renal transplant recipients because of Narrow Therapeutic Index and highly variable pharmacokinetics. The aim of this study was to detect inter‐patient pharmacokinetic variability of tacrolimus and to assess the predictability of individual tacrolimus concentrations at various times of the area under the curve (AUC) seeking to find the best sampling time to predict the exposure of tacrolimus in renal transplant recipients with triple therapy. This oral dose tacrolimus pharmacokinetics study was conducted in 18 Serbian renal transplant recipients on triple immunosuppressive therapy, including basiliximab. The first oral dose of tacrolimus (0.05 mg/kg) was given on day 5 post‐transplant; blood concentration was measured by microparticle enzyme immunoassay method. Associations between each sampling time‐point of concentrations and AUC12 were evaluated by Pearson correlation coefficients. Abbreviated sampling equations were derived by multiple, stepwise regression analyses. The variance in the strength of association between predicted AUC (AUCp) and AUC12 was reflected by linear regression coefficients. AUC12 showed remarkable inter‐individual variations after the first oral dose of tacrolimus. The area of the maximum AUC was four times higher than that of the minimum AUC. C4 seems to be an indicator of total body exposure to tacrolimus. Alternatively, the concentrations at 1.5, 4 and 8 hr as an abbreviated AUC were as good a predictor as a full pharmacokinetic study. Our results show a significant difference between men and women. A three‐point sampling method seemed to be the best abbreviated AUC for a cost‐effective tacrolimus monitoring strategy.