Telomerase activity in peripheral blood mononuclear cells from HIV and HIV–HCV coinfected patients

Replicative senescence of peripheral blood mononuclear cells (PBMC) plays an important role in the pathophysiology of chronic viral infections. Although there are controversial reports concerning telomerase activity in HIV monoinfected subjects, no data on HIV–HCV coinfected individuals is available...

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Bibliographic Details
Published in:Virus research 2010-02, Vol.147 (2), p.284-287
Main Authors: Reynoso, Rita, Laufer, Natalia, Bolcic, Federico, Quarleri, Jorge
Format: Article
Language:English
Subjects:
Adult
Blood - immunology
Cells, Cultured
Coinfection
Cross-Sectional Studies
Female
HCV
Hepacivirus - immunology
Hepatitis C - immunology
HIV
HIV Infections - immunology
HIV-1 - immunology
Human immunodeficiency virus 1
Humans
Leukocytes, Mononuclear - enzymology
Leukocytes, Mononuclear - virology
Male
Middle Aged
Telomerase - metabolism
Telomerase activity
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Summary:Replicative senescence of peripheral blood mononuclear cells (PBMC) plays an important role in the pathophysiology of chronic viral infections. Although there are controversial reports concerning telomerase activity in HIV monoinfected subjects, no data on HIV–HCV coinfected individuals is available. In this cross-sectional study telomerase activity was quantified in non-stimulated and mitogen-stimulated PBMC lysates from HIV-1 monoinfected and HIV–HCV coinfected individuals using real-time PCR. Up-regulation of telomerase activity after mitogen stimulation was impaired in PBMC of HIV monoinfected and HIV–HCV coinfected patients. The lack of an appropriate induction of this enzymatic activity after stimulus could partly account for immunosuppressive conditions observed in such patients.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2009.11.006