Hydrogen peroxide-induced apoptosis-like cell death in Entamoeba histolytica

• Published in: Parasitology international 2010-06, Vol.59 (2), p.166-172
• Main Authors: Ghosh, Anupama Sardar, Dutta, Suman, Raha, Sanghamitra
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis - drug effects; Apoptosis - physiology; Cell death; Cysteine Proteases - metabolism; Cysteine Proteinase Inhibitors - pharmacology; DNA Fragmentation; Entamoeba histolytica; Entamoeba histolytica - drug effects; Entamoeba histolytica - enzymology; Entamoeba histolytica - growth & development; Entamoeba histolytica - physiology; Flow Cytometry; Gastroenterology and Hepatology; Humans; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Infectious Disease; Leucine - analogs & derivatives; Leucine - pharmacology; Metazoa; Oxidative Stress - drug effects; Oxidative Stress - physiology; Reactive Oxygen Species - metabolism; Trophozoites - drug effects
• ISSN: 1383-5769; 1873-0329
• DOI: 10.1016/j.parint.2010.01.001

Abstract The microaerophilic intestinal parasitic protozoan Entamoeba histolytica has been previously shown to be highly susceptible to oxidative stress induced by hydrogen peroxide. However the mechanism of cell death was not investigated. Studies presented in this paper demonstrate several morphological features in the parasite when exposed to H2 O2 which are identical to metazoan apoptotic phenotype indicating a possible apoptosis-like cell death exhibited by E. histolytica in response to H2 O2 treatment. Trophozoite cell shrinkage, DNA fragmentation, phosphatidyl serine externalization and increased endogenous reactive oxygen species level have been observed in the protozoan parasite when exposed to 2.0 mM H2 O2 for different time periods. Although the parasite genome is completely devoid of any of the homologues of mammalian caspases it still codes for a huge number of cysteine proteases which may take over the apoptotic function of the caspases. But the present study indicates the existence of a cysteine protease independent programmed cell death in the parasite since E-64 the specific cysteine protease inhibitor could not rescue the cells from H2 O2 induced apoptosis-like cell death.