The use of COS-1 cells for studies of field and laboratory African swine fever virus samples

Different naturally occurring, cell adapted or genetically manipulated stocks of African swine fever virus were able to infect directly cultures of COS-1 cells, producing extensive cytopathic effects and amounts from 10 6 to 10 7 of infective progeny virus per ml. The induction of late virus-specifi...

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Bibliographic Details
Published in:Journal of virological methods 2010-03, Vol.164 (1), p.131-134
Main Authors: Hurtado, Carolina, Bustos, María José, Carrascosa, Angel L.
Format: Article
Language:English
Subjects:
African swine fever virus
African Swine Fever Virus - growth & development
African Swine Fever Virus - isolation & purification
African Swine Fever Virus - pathogenicity
Animals
ASFV
Biological and medical sciences
Cell sensitivity
Cercopithecus aethiops
COS Cells
COS-1
Cytopathogenic Effect, Viral
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Immunoblotting
Microbiology
Reverse Transcriptase Polymerase Chain Reaction
Techniques used in virology
Viral Plaque Assay
Viral Proteins - biosynthesis
Virology
Virus titration
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Summary:Different naturally occurring, cell adapted or genetically manipulated stocks of African swine fever virus were able to infect directly cultures of COS-1 cells, producing extensive cytopathic effects and amounts from 10 6 to 10 7 of infective progeny virus per ml. The induction of late virus-specific proteins, demonstrated by RT-PCR and immunoblotting, and the development of lysis plaques by all the virus samples tested so far, allowed the optimization of both titration and diagnostic assays, as well as the proposal of a method for selection of virus clones during the generation of virus mutants with specific gene deletions.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2009.11.030