Frequent Methylation of Vimentin in Well-differentiated Gastric Carcinoma

Background: Recently, it was shown that the vimentin gene, usually activated in mesenchymal cells, was highly methylated in colorectal carcinoma. Materials and Methods: The methylation status of the vimentin gene was examined in primary carcinomas and the corresponding normal tissues derived from 37...

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Bibliographic Details
Published in:Anticancer research 2009-06, Vol.29 (6), p.2227-2229
Main Authors: KITAMURA, Yo-Hei, SHIRAHATA, Atsushi, HIBI, Kenji, SAKATA, Makiko, GOTO, Tetsuhiro, MIZUKAMI, Hiroki, SAITO, Mitsuo, ISHIBASHI, Kazuyoshi, KIGAWA, Gaku, NEMOTO, Hiroshi, SANADA, Yutaka
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - secondary
Aged
Biological and medical sciences
Cell Differentiation
DNA Methylation
Female
Gastric Mucosa - metabolism
Gastric Mucosa - pathology
Gastroenterology. Liver. Pancreas. Abdomen
Gene Expression Regulation, Neoplastic
Humans
Lymphatic Metastasis
Male
Medical sciences
Neoplasm Staging
Polymerase Chain Reaction
Prognosis
Promoter Regions, Genetic
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
Vimentin - genetics
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Summary:Background: Recently, it was shown that the vimentin gene, usually activated in mesenchymal cells, was highly methylated in colorectal carcinoma. Materials and Methods: The methylation status of the vimentin gene was examined in primary carcinomas and the corresponding normal tissues derived from 37 patients with gastric carcinoma using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated. Results. Aberrant methylation of the vimentin gene was detected in 14 out of 37 (38%) primary gastric carcinomas. This result suggested that the aberrant methylation of the vimentin gene was frequent in gastric carcinomas. Subsequently, clinicopathological data were correlated with the methylation score. A significant difference was observed in histology (p=0.0429). In addition, a trend was shown toward advancement of gastric carcinomas with vimentin methylation (p=0.0588). Conclusion: In gastric carcinomas, well-differentiated adenocarcinoma was significantly methylated compared to poorly differentiated.
ISSN:0250-7005
1791-7530