Viral hemorrhagic septicemia and infectious pancreatic necrosis viruses replicate differently in rainbow trout gonad and induce different chemokine transcription profiles

Viral hemorrhagic septicemia virus (VHSV) and infectious pancreatic necrosis virus (IPNV) are two rainbow trout ( Oncorhynchus mykiss) pathogens. While IPNV is known to be vertically transmitted to the next generation through the oocyte, VHSV is known to replicate in the ovary and be transmitted hor...

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Bibliographic Details
Published in:Developmental and comparative immunology 2010-06, Vol.34 (6), p.648-658
Main Authors: Chaves-Pozo, Elena, Montero, Jana, Cuesta, Alberto, Tafalla, Carolina
Format: Article
Language:English
Subjects:
Animals
Birnaviridae Infections - immunology
Birnaviridae Infections - transmission
Birnaviridae Infections - virology
Cells, Cultured
Chemokines
Chemokines - genetics
Chemokines - metabolism
Female
Gene Expression Profiling
Gene Expression Regulation
Hemorrhagic Septicemia - immunology
Hemorrhagic Septicemia - transmission
Hemorrhagic Septicemia - virology
Immune response
Immunohistochemistry
Infectious pancreatic necrosis virus
Infectious pancreatic necrosis virus (IPNV)
Infectious pancreatic necrosis virus - pathogenicity
Infectious pancreatic necrosis virus - physiology
Novirhabdovirus - pathogenicity
Novirhabdovirus - physiology
Oncorhynchus mykiss
Organ Culture Techniques
Ovary
Ovary - immunology
Ovary - metabolism
Ovary - pathology
Viral hemorrhagic septicemia virus
Viral hemorrhagic septicemia virus (VHSV)
Virus Replication
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Summary:Viral hemorrhagic septicemia virus (VHSV) and infectious pancreatic necrosis virus (IPNV) are two rainbow trout ( Oncorhynchus mykiss) pathogens. While IPNV is known to be vertically transmitted to the next generation through the oocyte, VHSV is known to replicate in the ovary and be transmitted horizontally through the ovarian fluid. In this work, we wanted to study whether these differences had an effect on the immune response triggered in the ovary, with a focus on the chemokine response. We have studied the kinetics of viral gene expression and the sites of replication, confirming that great differences exist between the replication of the two viruses in the gonad. Next, we studied the levels of expression of several CXC and CC chemokines in the ovary and found that while VHSV strongly triggered chemokine transcription, IPNV had almost no effect. This lack of immune response might be an advantage that permits its vertical transmission.
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2010.01.009