Characterization and structural role of disulfide bonds in a highly knotted thionin from Pyrularia pubera

• Published in: Biopolymers 2005, Vol.80 (5), p.697-707
• Main Authors: Vila-Perelló, Miquel, Andreu, David
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amphipathic; Antimicrobial Cationic Peptides; cysteine-rich antimicrobial peptides; disulfide bonds; Disulfides - chemistry; Models, Molecular; Molecular Conformation; molecular dynamics; Molecular Sequence Data; partial reduction; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Protein Folding; proteolysis; Pyrularia - chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 0006-3525; 1097-0282
• DOI: 10.1002/bip.20270

Disulfide bonds play a crucial role in the stabilization of the amphipathic folding of the diverse families of cysteine‐rich antimicrobial peptides. The determination of cysteine pairings in these peptides has largely depended on sequence homology criteria, since the classical methods of disulfide bond characterization, which usually require proteolysis as a first step, encounter serious drawbacks derived from the tight folding and the presence of vicinal cysteines. We have chosen the Pyrularia pubera thionin, a 47‐residue peptide with four internal disulfides and a remarkable resistance to most proteases, as a representative member of this type of cysteine‐rich peptides and have shown that a combination of partial reduction and cyanylation readily allows the determination of its disulfide bonds. We have also studied by molecular dynamics and a combination of partial reduction and proteolysis the role of disulfide bonds in the stabilization of the tridimensional structure of this thionin and found a good agreement with our partial reduction data, suggesting that removal of only one disulfide bond is enough to significantly alter the folding of the peptide. © 2005 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 80: 697–707, 2005 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at [e-mail]