Structured Nucleic Acid Probes for Electrochemical Devices

The use of nucleic acid with a specific sequence and a highly ordered secondary structure such as hairpins, quadruplexes and pseudoknots as biological recognition elements and switches in biosensors is rapidly increasing because of their improved features (e.g. selectivity) when compared with the tr...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2009-10, Vol.21 (19), p.2077-2090
Main Authors: Miranda-Castro, Rebeca, de-los-Santos-Álvarez, Noemí, Lobo-Castañón, María Jesús, Miranda-Ordieres, Arturo J., Tuñón-Blanco, Paulino
Format: Article
Language:English
Subjects:
Aptamers
Biosensors
DNAzymes
Electrochemical transduction
Genosensor
Hairpin structure
Nucleic acids
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Summary:The use of nucleic acid with a specific sequence and a highly ordered secondary structure such as hairpins, quadruplexes and pseudoknots as biological recognition elements and switches in biosensors is rapidly increasing because of their improved features (e.g. selectivity) when compared with the traditional linear probes. Owing to the novelty, a critical outlook of their characteristics and a compilation of the latest advances are lacking. This article describes the potential of those nucleic acids probes whose molecular recognition ability relies on a conformational change (e.g. folding/unfolding mechanism) in electrochemical sensing. It provides an overview of the toolbox of assays using these probes for genosensors and aptasensors, highlighting its performance characteristics and the prospects and challenges for biosensor design.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.200904653