Determination of ferrous and ferric iron in aqueous biological solutions

A solvent extraction method was employed to determine ferrous and ferric iron in aqueous samples. Fe 3+ is selectively extracted into the organic phase ( n-heptane) using HDEHP (bis(2-ethylhexyl) hydrogen phosphate) and is then stripped using a strong acid. After separation, both oxidation states an...

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Bibliographic Details
Published in:Analytica chimica acta 2010-03, Vol.663 (2), p.172-177
Main Authors: Pepper, S.E., Borkowski, M., Richmann, M.K., Reed, D.T.
Format: Article
Language:English
Subjects:
Analytical chemistry
Applied sciences
Chelating Agents - chemistry
Chemical Fractionation
Chemistry
Citric Acid - chemistry
Edetic Acid - chemistry
Exact sciences and technology
Fe 2+ determination
Fe 3+ determination
Ferrozine method
Global environmental pollution
HDEHP extraction
Iron - analysis
Iron - metabolism
Ligands
Mass Spectrometry
Nitrilotriacetic Acid - chemistry
Oxidation-Reduction
Pollution
Shewanella - metabolism
Shewanella alga
Shewanella alga BrY
Solutions
Solvents - chemistry
Spectrometric and optical methods
Water Pollutants, Chemical - analysis
Water Pollutants, Chemical - metabolism
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Summary:A solvent extraction method was employed to determine ferrous and ferric iron in aqueous samples. Fe 3+ is selectively extracted into the organic phase ( n-heptane) using HDEHP (bis(2-ethylhexyl) hydrogen phosphate) and is then stripped using a strong acid. After separation, both oxidation states and the total iron content were determined directly by ICP-MS analysis. This extraction method was refined to allow determination of both iron oxidation states in the presence of strong complexing ligands, such as citrate, NTA and EDTA. The accuracy of the method was verified by crosschecking using a refinement of the ferrozine assay. Presented results demonstrate the ability of the extraction method to work in a microbiological system in the presence of strong chelating agents following the bioreduction of Fe 3+ by the Shewanella alga BrY. Based on the results we report, a robust approach was defined to separately analyze Fe 3+ and Fe 2+ under a wide range of potential scenarios in subsurface environments where radionuclide/metal contamination may coexist with strongly complexing organic contaminants.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2010.01.056