Investigation of recombinants of human influenza and fowl plague viruses

• Published in: Journal of general virology 1979-04, Vol.43 (1), p.183-191
• Main Authors: Ghendon, Y, Klimov, A, Blagoveshenskaya, O, Genkina, D
• Format: Article
• Language: English
• Subjects: animal diseases; animal health; Animals; Chick Embryo; Influenza A virus - analysis; Influenza A virus - genetics; Peptides - analysis; Recombination, Genetic; RNA, Viral - analysis; Viral Proteins - analysis; Virus Replication
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-43-1-183

Research Institute of Virus Preparations, Moscow, U.S.S.R. Recombinants of human influenza type A viruses, A/Krasnodar/101/1959 (H2N2) or A/Habarovsk/15/1976 (H3N2), and fowl plague virus (FPV), strain Weybridge (H av 1N eq 1) were obtained. The genome of the recombinant obtained by recombination of influenza A/Habarovsk/15/1976 virus and FPV contained the genes 4 (HA) and 6 (NA) derived from the influenza A/Habarovsk virus and all the other genes [1, 2, 3, 5 (NP), 7 (M), 8 (NS)] from FPV. The genome of the recombinant of A/Krasnodar/101/1959 virus and FPV contained the genes 2, 4 (HA) and 6 (NA) derived from influenza A/Krasnodar virus and all the other genes [1, 3, 5, (NP), 7 (M), 8 (NS)] from FPV. The recombinants, like FPV, gave high virus yields in chick embryos and could multiply at high temperatures (40 and 42 °C), but, like human influenza viruses, were non-pathogenic for chickens and did not replicate in chick embryo fibroblast culture, but did replicate in a human conjunctiva cell line, clone 1-5C-4. The virion transcriptase of the recombinants, in a number of properties determined in vitro , was similar to FPV transcriptase but not to the human influenza virus enzyme. Received 24 July 1978; accepted 25 October 1978.