Intra-abdominal administration of bevacizumab diminishes intra-peritoneal adhesions

Abstract Aim To determine the effect of a single dose of bevacizumab on adhesion formation in the rat cecum abrasion model. Methods The cecum and parietal peritoneum of 38 male Wistar rats were abraded to promote adhesion formation. The rats were randomized into 2 groups: group 1 received bevacizuma...

Full description

Saved in:
Bibliographic Details
Published in:The American journal of surgery 2010-08, Vol.200 (2), p.270-275
Main Authors: Ignjatovic, Dejan, Aasland, Kristine, Pettersen, Marianne, Sund, Stale, Chen, Yin, Spasojevic, Milan, Nesgaard, Jens Marius
Format: Article
Language:English
Subjects:
Abdomen
Abrasion
Adhesion
Angiogenesis
Animals
Anti-Inflammatory Agents - administration & dosage
Antibodies, Monoclonal - administration & dosage
Antibodies, Monoclonal, Humanized
Bevacizumab
Catheters
Cecum
Comparative animal study
Cytokines
Dehiscence
Disease Models, Animal
Group dynamics
Histopathology
Immunotherapy
Injections, Intraperitoneal
Laboratory animals
Laparoscopy
Male
Monoclonal antibodies
Pain
Peritoneum
Permeability
Postoperative
Quality
Rats
Rats, Wistar
Rodents
Statistical analysis
Statistical methods
Surgery
Tissue Adhesions - diagnosis
Tissue Adhesions - prevention & control
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - antagonists & inhibitors
Wistar rats
Wounds
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Aim To determine the effect of a single dose of bevacizumab on adhesion formation in the rat cecum abrasion model. Methods The cecum and parietal peritoneum of 38 male Wistar rats were abraded to promote adhesion formation. The rats were randomized into 2 groups: group 1 received bevacizumab (2.5 mg/kg) intraperitoneally, and group 2 received saline. On day 30 animals were killed, adhesions scored, and histopathological samples taken. Results There was no wound dehiscence; there were 2 incision hernias (5.3%), 1 per group. Thirty-seven animals developed adhesions (97.4%). Adhesion grade and severity scores were significantly different between groups 1 and 2 at 2.7:1.6 ( P = .018) and 3.8:2.7 ( P = .007), respectively. There was no difference in adhesion square area (27.7:25.0%; P = .16), location ( P = 1.00), or number (2.1:1.3; P = .06). Histopathology confirmed the statistical difference between groups ( P = .049), and a highly significant correlation between results was shown ( r = .758; P = .0001). Conclusion A single dose of intraperitoneal bevacizumab significantly reduces grade and severity of abdominal adhesions in the cecum abrasion rat model.
ISSN:0002-9610
1879-1883
DOI:10.1016/j.amjsurg.2009.08.038