Complexation of Europium(III) with the Zwitterionic Form of Amino Acids Studied with Ultraviolet—Visible and Time-Resolved Laser-Induced Fluorescence Spectroscopy

• Published in: Applied spectroscopy 2010-08, Vol.64 (8), p.930-935
• Main Authors: Heller, Anne, Rönitz, Olivia, Barkleit, Astrid, Bernhard, Gert, Ackermann, Jörg-Uwe
• Format: Article
• Language: English
• Subjects: Amino Acids - chemistry; Europium - chemistry; Hydrogen-Ion Concentration; Ions - chemistry; Metals, Heavy - chemistry; Spectrometry, Fluorescence - methods; Spectrophotometry, Ultraviolet - methods; Temperature; Time Factors
• ISSN: 0003-7028; 1943-3530
• DOI: 10.1366/000370210792081127

The complex formation of europium(III) with the zwitterionic form of amino acids (alanine, phenylalanine, and threonine) has been studied in aqueous solution. Measurements were performed at I = 0.1 M (NaCl/NaClO4), room temperature, and trace metal concentrations in the range of pH 2 to 8 using ultraviolet–visible (UV-Vis) and time-resolved laser-induced fluorescence spectroscopy (TRLFS). While complexation leads to a significant luminescence enhancement in the emission spectrum of the metal ion, absorption in the UV-Vis spectrum of the amino acid (AA) decreases. As zwitterionic species (AAH), all three ligands form weak complexes with 1:1 stoichiometry and a general formula of EuAAH3+ with the metal. The complex stability constants were determined to be log K ∼ 1 for all complexes, indicating the negligible contribution of the amino acid side chain to the complex formation reaction.