Comparison of low-density lipoprotein cholesterol concentrations measured by a direct homogeneous assay and by the Friedewald formula in a large community population

We compare the direct homogeneous low-density lipoprotein cholesterol (LDL-C) assay with the Friedewald formula (FF) for determination of LDL-C in a large community-dwelling population. A total of 21,194 apparently healthy subjects aged 40 to 79 years with triglyceride (TG) concentrations < 4.52...

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Bibliographic Details
Published in:Clinica chimica acta 2010-11, Vol.411 (21), p.1774-1780
Main Authors: Tanno, Kozo, Okamura, Tomonori, Ohsawa, Masaki, Onoda, Toshiyuki, Itai, Kazuyoshi, Sakata, Kiyomi, Nakamura, Motoyuki, Ogawa, Akira, Kawamura, Kazuko, Okayama, Akira
Format: Article
Language:English
Subjects:
Adult
Aged
Bias
Cholesterol, LDL - blood
Community-based study
Enzymatic homogeneous LDL-C assay
Epidemiologic Measurements
Fasting
Female
Friedewald formula
Humans
Japanese
Low-density lipoprotein cholesterol
Male
Methods
Middle Aged
Regression Analysis
Reproducibility of Results
Residence Characteristics
Triglyceride
Triglycerides - blood
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Summary:We compare the direct homogeneous low-density lipoprotein cholesterol (LDL-C) assay with the Friedewald formula (FF) for determination of LDL-C in a large community-dwelling population. A total of 21,194 apparently healthy subjects aged 40 to 79 years with triglyceride (TG) concentrations < 4.52 mmol/l were enrolled. LDL-C were directly measured by the enzymatic homogeneous assay (LDL-C (D)) and also estimated by the FF (LDL-C (F)). Paired t-test, Pearson's correlation coefficient and linear regression analysis were performed and the concordances of the National Cholesterol Education Program (NCEP) risk category were estimated. Both in fasting (n = 3270) and nonfasting samples (n = 17,924), LDL-C (D) highly correlated with LDL-C (F): r = 0.971 and 0.955, respectively. Concordant results for NCEP categories were 84.8% for fasting samples and 80.1% for nonfasting samples. However, the bias between the 2 measurements increased in samples with TG concentrations > 1.69 mmol/l, especially in nonfasting samples. The results showing less variability of the direct LDL-C assay than that of the FF in nonfasting samples suggest that epidemiological studies can use LDL-C measured by the direct assay both in fasting and nonfasting samples.
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2010.07.034