Quantum Dots Bearing Lectin-Functionalized Nanoparticles as a Platform for In Vivo Brain Imaging

Delivery of imaging agents to the brain is highly important for the diagnosis and treatment of central nervous system (CNS) diseases, as well as the elucidation of their pathophysiology. Quantum dots (QDs) provide a novel probe with unique physical, chemical, and optical properties, and become a pro...

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Bibliographic Details
Published in:Bioconjugate chemistry 2008-11, Vol.19 (11), p.2189-2195
Main Authors: Gao, Xiaoling, Chen, Jun, Chen, Jiyao, Wu, Bingxian, Chen, Hongzhuan, Jiang, Xinguo
Format: Article
Language:English
Subjects:
Administration, Intranasal
Animals
Biochemistry
Brain
Brain - metabolism
Diagnostic Imaging - instrumentation
Diagnostic Imaging - methods
Feasibility Studies
Medical imaging
Mice
Mice, Inbred BALB C
Nanoparticles
Neurological disorders
Particle Size
Pathology
Protein Stability
Quantum Dots
Tissue Distribution
Wheat Germ Agglutinins - administration & dosage
Wheat Germ Agglutinins - chemistry
Wheat Germ Agglutinins - metabolism
Wheat Germ Agglutinins - pharmacokinetics
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Summary:Delivery of imaging agents to the brain is highly important for the diagnosis and treatment of central nervous system (CNS) diseases, as well as the elucidation of their pathophysiology. Quantum dots (QDs) provide a novel probe with unique physical, chemical, and optical properties, and become a promising tool for in vivo molecular and cellular imaging. However, their poor stability and low blood−brain barrier permeability severely limit their ability to enter into and act on their target sites in the CNS following parenteral administration. Here, we developed a QDs-based imaging platform for brain imaging by incorporating QDs into the core of poly(ethylene glycol)−poly(lactic acid) nanoparticles, which was then functionalized with wheat germ agglutinin and delivered into the brain via nasal application. The resulting nanoparticles, with high payload capacity, are water-soluble, stable, and showed excellent and safe brain targeting and imaging properties. With PEG functional terminal groups available on the nanoparticles surface, this nanoprobe allows for conjugation of various biological ligands, holding considerable potential for the development of specific imaging agents for various CNS diseases.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc8002698