Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment

• Published in: Vaccine 2010-03, Vol.28 (10), p.2201-2206
• Main Authors: Segat, Ludovica, Brandão, Lucas A.C, Guimarães, Rafael L, Pontillo, Alessandra, Athanasakis, Emmanouil, de Oliveira, Rafael Martins, Arraes, Luiz C, de Lima Filho, Josè Luiz, Crovella, Sergio
• Format: Article
• Language: English
• Subjects: AIDS Vaccines - immunology; Allergy and Immunology; Applied microbiology; Biological and medical sciences; Dendritic cell immunotherapy; Dendritic cells; Dendritic Cells - immunology; Ethnic Groups; Fundamental and applied biological sciences. Psychology; Gene Frequency; Genes; Genetic engineering; Genomes; Genotype; Genotype & phenotype; Haplotypes; HIV; HIV infection; HIV Infections - therapy; Human immunodeficiency virus; Human viral diseases; Humans; Immune response; Immune response (cell-mediated); Immune system; Immunity; Immunity, Innate - genetics; Immunotherapy - methods; Infectious diseases; Innate immunity; Mannose-Binding Lectin - genetics; Medical sciences; Microbiology; Minority & ethnic groups; Miscellaneous; Modulation; Nitric Oxide Synthase Type I - genetics; Nitric-oxide synthase; Polymorphism, Single Nucleotide; Progressions; Single-nucleotide polymorphism; Vaccination; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Viral infections; Virology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2009.12.056

Abstract Dendritic cells (DCs)-based vaccine was demonstrated to increase HIV specific cellular immune response; however, in some HIV-infected patients, the response to the vaccine resulted to be not effective. In order to understand if the outcome of the vaccination may be influenced by the host's genome and natural immunity, we studied the innate immune genome of HIV-infected patients previously vaccinated with DCs. We identified 15 SNPs potentially associated with the response to the immuno-treatment and two SNPs significantly associated with the modulation of the response to the DC vaccine: MBL2 rs10824792 and NOS1 rs693534. These two SNPs were also studied in different ethnic groups (Brazilians, African and Caucasian) of HIV-infected, exposed uninfected and unexposed uninfected subjects. The HIV positive Caucasian patients were also characterized by different disease progressions. Our findings suggest that, independently and/or in addition to other variables, the host's genome could significantly contribute to the modulation of the response to the DC vaccine.