Horse Prion Protein NMR Structure and Comparisons with Related Variants of the Mouse Prion Protein

• Published in: Journal of molecular biology 2010-07, Vol.400 (2), p.121-128
• Main Authors: Pérez, Daniel R., Damberger, Fred F., Wüthrich, Kurt
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; cellular horse prion protein; Equus caballus; Horses; Humans; Mice; Mice, Transgenic; Models, Molecular; Molecular Sequence Data; NMR structure determination; Nuclear Magnetic Resonance, Biomolecular; Prion Diseases - genetics; Prion Diseases - metabolism; Prions - chemistry; Prions - genetics; Prions - metabolism; Protein Isoforms - chemistry; Protein Isoforms - genetics; Protein Isoforms - metabolism; protein structure; Protein Structure, Secondary; Sequence Alignment; transmissible spongiform encephalopathy; β2–α2 loop
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1016/j.jmb.2010.04.066

The NMR structure of the horse ( Equus caballus) cellular prion protein at 25 °C exhibits the typical PrP C [cellular form of prion protein (PrP)] global architecture, but in contrast to most other mammalian PrP Cs, it contains a well-structured loop connecting the β2 strand with the α2 helix. Comparison with designed variants of the mouse prion protein resulted in the identification of a single amino acid exchange within the loop, D167S, which correlates with the high structural order of this loop in the solution structure at 25 °C and is unique to the PrP sequences of equine species. The β2–α2 loop and the α3 helix form a protein surface epitope that has been proposed to be the recognition area for a hypothetical chaperone, “protein X,” which would promote conversion of PrP C into the disease-related scrapie form and thus mediate intermolecular interactions related to the transmission barrier for transmissible spongiform encephalopathies (TSEs) between different species. The present results are evaluated in light of recent indications from in vivo experiments that the local β2–α2 loop structure affects the susceptibility of transgenic mice to TSEs and the fact that there are no reports on TSE in horses.