A finite element model predicts the mechanotransduction response of tendon cells to cyclic tensile loading

The importance of fluid-flow-induced shear stress and matrix-induced cell deformation in transmitting the global tendon load into a cellular mechanotransduction response is yet to be determined. A multiscale computational tendon model composed of both matrix and fluid phases was created to examine h...

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Bibliographic Details
Published in:Biomechanics and modeling in mechanobiology 2008-10, Vol.7 (5), p.405-416
Main Authors: Lavagnino, Michael, Arnoczky, Steven P., Kepich, Eugene, Caballero, Oscar, Haut, Roger C.
Format: Article
Language:English
Subjects:
Animals
Bioengineering
Biological and Medical Physics
Biomechanics
Biomedical Engineering and Bioengineering
Biophysics
Cells, Cultured
Culture Media
Engineering
Finite Element Analysis
Fluid flow
Gene expression
Mechanotransduction, Cellular - physiology
Models, Biological
Original Paper
Orthopedics
Rats
Rats, Sprague-Dawley
Shear stress
Space life sciences
Strain
Stress, Mechanical
Tendons - cytology
Tendons - metabolism
Tendons - physiology
Tensile strength
Theoretical and Applied Mechanics
Weight-Bearing - physiology
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Summary:The importance of fluid-flow-induced shear stress and matrix-induced cell deformation in transmitting the global tendon load into a cellular mechanotransduction response is yet to be determined. A multiscale computational tendon model composed of both matrix and fluid phases was created to examine how global tendon loading may affect fluid-flow-induced shear stresses and membrane strains at the cellular level. The model was then used to develop a quantitative experiment to help understand the roles of membrane strains and fluid-induced shear stresses on the biological response of individual cells. The model was able to predict the global response of tendon to applied strain (stress, fluid exudation), as well as the associated cellular response of increased fluid-flow-induced shear stress with strain rate and matrix-induced cell deformation with strain amplitude. The model analysis, combined with the experimental results, demonstrated that both strain rate and strain amplitude are able to independently alter rat interstitial collagenase gene expression through increases in fluid-flow-induced shear stress and matrix-induced cell deformation, respectively.
ISSN:1617-7959
1617-7940
DOI:10.1007/s10237-007-0104-z