FIRST REPORT OF 'CANDIDATUS LIBERIBACTER AMERICANUS' TRANSMISSION FROM MURRAYA PANICULATA TO SWEET ORANGE BY DIAPHORINA CITRI

Huanglongbing (HLB), one of the most destructive citrus diseases in the world was first reported from Brazil in 2004. 'Candidates Liberibacter asiaticus' and 'Candidatus Liberibacter americanus' were detected (Teixeira et al., 2005), both transmitted by the Asian citrus psyllid D...

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Bibliographic Details
Published in:Journal of plant pathology 2010-07, Vol.92 (2), p.546-546
Main Authors: Gasparoto, M.C.G., Bassanezi, R.B., Amorim, L., Montesino, L.H., Lourenço, S.A., Wulff, N.A., Teixeira, D.C., Mariano, A.G., Martins, E.C., Leite, A.P.R., Filho, A. Bergamin
Format: Article
Language:English
Subjects:
Citrus
Citrus sinensis
Diaphorina citri
Disease Note
Murraya paniculata
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Summary:Huanglongbing (HLB), one of the most destructive citrus diseases in the world was first reported from Brazil in 2004. 'Candidates Liberibacter asiaticus' and 'Candidatus Liberibacter americanus' were detected (Teixeira et al., 2005), both transmitted by the Asian citrus psyllid Diaphorina citri (Yamamoto et al., 2006). Citrus spp. and the ornamental shrub Murraya paniculata are hosts of D. citri and 'Ca. Liberibacter americanus' (Lopes et al., 2005). However, D. citri transmission of this pathogen from M. paniculata to citrus has not yet been proved. Psyllids free from 'Ca. Liberibacter spp.' were reared in M. paniculata plants free from 'Ca. Liberibacter spp.', Two potted plants of M. paniculata graft-inoculated with 'Ca. Liberibacter americanus' were placed in the center of an insect-proof screenhouse and surrounded by 158 healthy cv. Valencia sweet orange potted plants. From April 2008 and during 30 months, 100 adult laboratory-reared psyllids, free from 'Ca. Liberibacter spp.', were confined monthly on infected M. paniculata plants. After seven days acquisition access period, the psyllids were set free and allowed to move freely inside the screenhouse. Every three months after the first psyllid liberation, leaf samples of all plants were collected to detect the presence of 'Ca. Liberibacter americanus'. All psyllid and leaf samples were assayed by conventional PCR and qPCR. From July 2008 onwards, the pathogen was detected in the psyllids. In February 2009 'Ca. Liberibacter americanus' was detected in eight plants and in August in two additional plants. All PCR-positive plants showed typical HLB symptoms in September 2009. These results show that D. citri can acquire 'Ca. Liberibacter americanus' from M. paniculata and transmit it to citrus plants.
ISSN:1125-4653
2239-7264