Structural characterization of glycoprotein NGAL, an early predictive biomarker for acute kidney injury

Neutrophil gelatinase-associated lipocalin (NGAL) is a promising new renal biomarker that can reduce the time to diagnose acute kidney injury (AKI). There is little information available about complex glycans on NGAL. Detailed structural characterization of NGAL is necessary to understand the struct...

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Bibliographic Details
Published in:Carbohydrate research 2010-10, Vol.345 (15), p.2252-2261
Main Authors: Zhao, Cheng, Ozaeta, Panfilo, Fishpaugh, Jeffrey, Rupprecht, Kevin, Workman, Ryan, Grenier, Frank, Ramsay, Carol
Format: Article
Language:English
Subjects:
Acute Kidney Injury - diagnosis
Acute Kidney Injury - urine
Amino Acid Sequence
Animals
Biomarker
CHO Cells
Cricetinae
Cricetulus
Glycoprotein
Glycoproteins - chemistry
Glycoproteins - urine
Humans
Mass spectrometry
Molecular Sequence Data
N-Glycans
NGAL
Structural characterization
Tandem Mass Spectrometry
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Summary:Neutrophil gelatinase-associated lipocalin (NGAL) is a promising new renal biomarker that can reduce the time to diagnose acute kidney injury (AKI). There is little information available about complex glycans on NGAL. Detailed structural characterization of NGAL is necessary to understand the structural variability of NGAL used as a standard in the NGAL immunoassay. This study demonstrated that 7–9% of mutant (C87S) recombinant NGAL was N-glycosylated and no O-glycosylation was detected. The NGAL sequence was confirmed by nanoLC/MS/MS following in gel and in solution trypsin digestion, and the N-glycosylation site was localized by MS/MS. Six different mutant recombinant NGAL samples (samples A–F) were analyzed in this study; however, these samples demonstrated two different glycan patterns. Forty-one N-glycans were detected in sample A and the more abundant N-glycans were unsialylated. Forty-three N-glycans were detected in sample F and the more abundant N-glycans were sialylated. Each of the other four samples (B–E) had a similar N-glycan pattern as sample F.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2010.07.024