MCP-1 Production in Temporomandibular Joint Inflammation

• Published in: Journal of dental research 2010-10, Vol.89 (10), p.1117-1122
• Main Authors: Ogura, N., Satoh, K., Akutsu, M., Tobe, M., Kuyama, K., Kuboyama, N., Sakamaki, H., Kujiraoka, H., Kondoh, T.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Animals; Anthracenes - pharmacology; Autoantigens - analysis; Autoantigens - drug effects; Biological and medical sciences; Calcium-Calmodulin-Dependent Protein Kinases - antagonists & inhibitors; Cell Culture Techniques; Chemokine CCL2 - analysis; Chemokine CCL2 - drug effects; Cytokines - analysis; Cytokines - drug effects; Dentistry; Diseases of the osteoarticular system; Female; Flavonoids - pharmacology; Humans; Inflammatory joint diseases; Interleukin-1beta - antagonists & inhibitors; Interleukin-1beta - pharmacology; JNK Mitogen-Activated Protein Kinases - antagonists & inhibitors; Joint Dislocations - immunology; Male; Medical sciences; Microarray Analysis; Miscellaneous. Osteoarticular involvement in other diseases; NF-kappa B - antagonists & inhibitors; Osteoarthritis - immunology; Rats; Rats, Inbred Lew; RNA, Messenger - analysis; Synovial Fluid - chemistry; Synovial Fluid - drug effects; Synovial Fluid - immunology; Synovial Membrane - drug effects; Synovial Membrane - immunology; Synovitis - immunology; Temporomandibular Joint Disc - immunology; Temporomandibular Joint Disorders - immunology; Young Adult
• ISSN: 0022-0345; 1544-0591
• DOI: 10.1177/0022034510376041

Synovitis, which is characterized by the infiltration of inflammatory cells, often accompanies progression of temporomandibular joint disorder (TMD) symptoms. Because IL-1β is elevated in synovial fluids obtained from TMDs, we hypothesized that IL-1β-responsive genes in synoviocytes may help identify the putative genes associated with synovitis. Using microarray analysis, we found that monocyte chemoattractant protein-1 (MCP-1) mRNA levels were elevated in IL-1β-stimulated synoviocytes. MCP-1 is a member of the chemokine superfamily. The production of MCP-1 was increased in synoviocytes treated with IL-1β. When IL-1β was injected into the cavities of rat TMJs, inflammatory cells and MCP-1-positive cells were detected in the synovial tissues. Furthermore, MCP-1 levels were higher in synovial fluids from individuals with pain compared with those without pain. Inhibitors of MAP-kinases and NF-κB reduced IL-1β-induced MCP-1 production. These results suggest that MCP-1 stimulated by IL-1β is one of the factors associated with the inflammatory progression of TMDs.