Mapping and SNP analysis of bovine candidate genes for meat and carcass quality

Summary The chromosomal localization of 13 bovine genes was determined using radiation hybrid (RH) mapping. The RH mapping data were in agreement with published data using either linkage, somatic cell hybrids or in situ hybridization. Mutation analysis using single‐stranded conformational polymorphi...

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Bibliographic Details
Published in:Animal genetics 2003-10, Vol.34 (5), p.349-353
Main Authors: Haegeman, A., Williams, J. L., Law, A., Van Zeveren, A., Peelman, L. J.
Format: Article
Language:English
Subjects:
Animals
Cattle - genetics
Cattle - physiology
DNA Mutational Analysis
DNA Primers
Gene Frequency
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide - genetics
Polymorphism, Single Nucleotide - physiology
Radiation Hybrid Mapping
RFLP
Sequence Analysis, DNA
SNP
SSCP
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Summary:Summary The chromosomal localization of 13 bovine genes was determined using radiation hybrid (RH) mapping. The RH mapping data were in agreement with published data using either linkage, somatic cell hybrids or in situ hybridization. Mutation analysis using single‐stranded conformational polymorphism, restriction fragment length polymorphism (RFLP) and sequencing revealed 13 SNPs in four different genes, namely carboxypeptidase E (CPE), uncoupling protein 2 (UCP2), single‐minded (Drosophila) homologue 1 (SIM1) and methallothionein IIa (MT2A). With the exception of one mutation in CPE, all other mutations are either silent or are situated in an intron. The polymerase chain reaction RFLP was used on unrelated animals from different cattle breeds for determing allelic distribution.
ISSN:0268-9146
1365-2052
DOI:10.1046/j.1365-2052.2003.01008.x