The significance of N-linked glycosylation in pig endogenous retrovirus infectivity

The significance of the envelope glycoprotein in the transmission of pig endogenous retrovirus (PERV) to human cells was investigated. Pig endothelial cells (PEC) were transduced with the LacZ gene by a pseudotype infection and then infected with PERV subtype B. Culture supernatants of the infected...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-10, Vol.310 (2), p.327-333
Main Authors: Hazama, Kenji, Miyagawa, Shuji, Miyazawa, Takayuki, Yamada, Junko, Tomonaga, Keizo, Ota, Mitsunori, Matsuda, Hikaru, Shirakura, Ryota
Format: Article
Language:English
Subjects:
alpha-Glucosidases - pharmacology
Animals
Cell Line
Concanavalin A - pharmacology
Endogenous Retroviruses - drug effects
Endogenous Retroviruses - metabolism
Endogenous Retroviruses - pathogenicity
Endothelium - virology
Glycosylation - drug effects
Humans
Mannose
Mannosidases - pharmacology
Membrane Glycoproteins - metabolism
Morpholines - pharmacology
N-linked sugar
Pig endogenous retrovirus
Reverse Transcriptase Polymerase Chain Reaction
Swine
Tunicamycin - pharmacology
Viral Envelope Proteins - metabolism
Xenotransplantation
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Summary:The significance of the envelope glycoprotein in the transmission of pig endogenous retrovirus (PERV) to human cells was investigated. Pig endothelial cells (PEC) were transduced with the LacZ gene by a pseudotype infection and then infected with PERV subtype B. Culture supernatants of the infected PEC previously incubated with several types of drugs were inoculated into HEK293 cells. The inoculated cells were then stained and the number of LacZ-positive foci was counted. PERV from tunicamycin treated PEC was not transmitted to human cells, indicating the importance of N-linked sugars in this process. Moreover, while inhibition of the terminal α-glucose residues from the precursor N-glycan by castanospermine and 1-deoxynojirimycin attenuated PERV infectivity, the mannosidase inhibitors, 1-deoxymannojirimycin and swainsonine, upregulated the infectivity. In addition, treatment with α-mannosidase and incubation with concanavalin A completely abrogated the transmission of PERV to HEK293. These data imply that the high-mannose type of N-glycan plays a key role in PERV infectivity.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2003.08.142