Characterization of Enterococcus faecium mutants resistant to mundticin KS, a class IIa bacteriocin

1 National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan 2 Research Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan Correspondence Jun Shima shima...

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Published in:Microbiology (Society for General Microbiology) 2003-10, Vol.149 (10), p.2901-2908
Main Authors: Sakayori, Youko, Muramatsu, Mizuho, Hanada, Satoshi, Kamagata, Yoichi, Kawamoto, Shinichi, Shima, Jun
Format: Article
Language:English
Subjects:
Bacteriocins - pharmacology
cardiolipin
Drug Resistance, Bacterial
Enterococcus faecium
Enterococcus faecium - drug effects
Fatty Acids - analysis
Magnesium - pharmacology
Membrane Lipids - analysis
mundticin KS
Mutation
phosphatidylglycerol
Phospholipids - analysis
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Summary:1 National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan 2 Research Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan Correspondence Jun Shima shimaj{at}nfri.affrc.go.jp The emergence and spread of mutants resistant to bacteriocins would threaten the safety of using bacteriocins as food preservatives. To determine the physiological characteristics of resistant mutants, mutants of Enterococcus faecium resistant to mundticin KS, a class IIa bacteriocin, were isolated. Two types of mutant were found that had different sensitivities to other antimicrobial agents such as nisin (class I) and kanamycin. Both mutants were resistant to mundticin KS even in the absence of Mg 2+ ions. The composition of unsaturated fatty acids in the resistant mutants was significantly increased in the presence of mundticin KS. The composition of the phospholipids in the two resistant mutants also differed from those in the wild-type strain. The putative zwitterionic amino-containing phospholipid in both mutants significantly increased, whereas amounts of phosphatidylglycerol and cardiolipin decreased. These changes in membrane structure may influence resistance of enterococci to class IIa and class I bacteriocins. Abbreviations: ACP, amino-containing phospholipid; CL, cardiolipin; CPA, cyclopropanic acid; PG, phosphatidylglycerol
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.26435-0