Size and Stability of Reconstituted Sesame Oil Bodies

• Published in: Biotechnology progress 2003, Vol.19 (5), p.1623-1626
• Main Authors: Peng, Chi-Chung, Lin, I-Ping, Lin, Ching-Kuan, Tzen, Jason T. C.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; genipin; glutaraldehyde; Hydrogen-Ion Concentration; Inclusion Bodies - metabolism; Inclusion Bodies - ultrastructure; oleosins; proteinase K; Seeds - cytology; Seeds - metabolism; sesame oil; Sesame Oil - chemistry; Sesame Oil - isolation & purification; Sesame Oil - metabolism; Sesamum - cytology; Sesamum - metabolism; Sesamum indicum; Surface Properties; Temperature; Triglycerides
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1021/bp034129z

Oil bodies of sesame seeds comprise a triacylglycerol matrix, which is surrounded by a monolayer of phospholipids embedded with unique proteins, mainly structural proteins termed oleosins. Artificial oil bodies were successfully reconstituted with various compositions of triacylglycerols, phospholipids, and oil‐body proteins. The sizes of reconstituted oil bodies displayed a normal distribution with an average size proportional to the ratio of triacylglycerols to oil‐body proteins. Both thermostability and structural stability of reconstituted oil bodies decreased as their sizes increased, and vice versa. Proteinase K digestion indicated that oleosins anchored both native and reconstituted oil bodies via their central hydrophobic domains. The stability of reconstituted oil bodies, as well as the purified ones from sesame seeds, could be substantially enhanced after their surface proteins were cross‐linked by glutaraldehyde or genipin.