IL-10 is critical for Th2 responses in a murine model of allergic dermatitis

We found that mechanical injury to mouse skin, which can be caused by tape stripping, results in rapid induction of IL-10 mRNA. IL-10-/- mice were used to examine the role of IL-10 in a mouse model of allergic dermatitis induced by epicutaneous (EC) sensitization with OVA on tape-stripped skin. Skin...

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Bibliographic Details
Published in:The Journal of clinical investigation 2003-10, Vol.112 (7), p.1058-1066
Main Authors: Laouini, Dhafer, Alenius, Harri, Bryce, Paul, Oettgen, Hans, Tsitsikov, Erdyni, Geha, Raif S
Format: Article
Language:English
Subjects:
Animals
Antigen-Presenting Cells - physiology
Antigens
Biomedical research
Chemokine CCL11
Chemokines, CC - genetics
Cytokines
Dendritic Cells - immunology
Dermatitis
Dermatitis, Atopic - immunology
Eosinophils - physiology
Immunization
Infections
Inflammation
Interferon-gamma - genetics
Interleukin-10 - genetics
Interleukin-10 - physiology
Interleukin-4 - genetics
Interleukin-5 - genetics
Laboratories
Lavage
Listeria
Lymphocytes
Mice
Mice, Inbred C57BL
Ovalbumin - immunology
Proteins
Protozoa
RNA, Messenger - analysis
T cell receptors
Th1 Cells - physiology
Th2 Cells - physiology
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Summary:We found that mechanical injury to mouse skin, which can be caused by tape stripping, results in rapid induction of IL-10 mRNA. IL-10-/- mice were used to examine the role of IL-10 in a mouse model of allergic dermatitis induced by epicutaneous (EC) sensitization with OVA on tape-stripped skin. Skin infiltration by eosinophils and expression of eotaxin, IL-4, and IL-5 mRNA in OVA-sensitized skin sites were severely diminished in IL-10-/- mice. Following in vitro stimulation with OVA, splenocytes from EC-sensitized IL-10-/- mice secreted significantly less IL-4, but significantly more IFN-gamma, than splenocytes from WT controls. A similar skewing in cytokine secretion profile was observed in the splenocytes of IL-10-/- mice immunized intraperitoneally with OVA. IL-10-/- APCs skewed the in vitro response of OVA T cell receptor (TCR) transgenic T cells towards Th1. Examination of the Th response of WT and IL-10-/- mice immunized with OVA-pulsed WT or IL-10-/- DCs revealed that both DCs and T cells participate in IL-10 skewing of the Th2 response in vivo. These results suggest that IL-10 plays an important role in the Th2 response to antigen and in the development of skin eosinophilia in a murine model of allergic dermatitis.
ISSN:0021-9738
1558-8238
DOI:10.1172/jci18246