cDNA cloning and characterization of the nuclear gene encoding chloroplast glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa

Using a PCR-generated homologous probe, cDNA was recovered (GapA cDNA) encoding the complete 338 amino-acid chloroplast GAPDH of the marine red alga Gracilaria verrucosa, together with its 78 amino- acid transit peptide. This cDNA was readily aligned with chloroplast-localized GAPDH genes (GapA and...

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Bibliographic Details
Published in:Current genetics 1993-05, Vol.23 (5-6), p.483-489
Main Authors: Zhou, Y.H. (National Research Council of Canada, Halifax, Nova Scotia (Canada). Inst. for Marine Biosciences), Ragan, M.A
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Blotting, Southern
cDNA
Cell Nucleus
Chloroplast isoenzyme
CHLOROPLASTE
Chloroplasts - enzymology
Cloning, Molecular
CLOROPLASTO
DNA
EVOLUCION
EVOLUTION
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes. Genome
Glyceraldehyde-3-phosphate dehydrogenase
Glyceraldehyde-3-Phosphate Dehydrogenases - genetics
Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism
GRACILARIA
Gracilaria verrucosa
ISOENZIMAS
ISOENZYME
Molecular and cellular biology
Molecular evolution
Molecular genetics
Molecular Sequence Data
Polymerase Chain Reaction
Rhodophyta - enzymology
Rhodophyta - genetics
Sequence Homology, Amino Acid
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Description
Summary:Using a PCR-generated homologous probe, cDNA was recovered (GapA cDNA) encoding the complete 338 amino-acid chloroplast GAPDH of the marine red alga Gracilaria verrucosa, together with its 78 amino- acid transit peptide. This cDNA was readily aligned with chloroplast-localized GAPDH genes (GapA and GapB) of green plants. The proline residue which contributes to the specificity of NAD(+) binding to cytosolic GAPDHs is absent from the deduced polypeptide chain of G. verrucosa GapA as is also the case in the chloroplast GAPDHs of plants. The transit peptide shows a high proportion of random coil, an amino-terminal Met-Ala dipeptide, a high content of hydroxylamino acids, and a net positive charge. The polyadenylation signal appears to be AGTAAA. Genomic Southern-hybridization data indicate that only one chloroplast-GAPDH gene may occur in G. verrucosa. Bootstrapped parsimony trees indicate that the G. verrucosa GapA gene is a sister group to plant chloroplast-GAPDH genes, and are most readily interpreted as showing that red algal and plant chloroplast-localized GAPDHs arose in a single endosymbiotic event.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00312639