Recognition of Leuconostoc oenos strains by the use of DNA restriction profiles

The chromosome of 41 Leuconostoc oenos strains obtained from collections in different countries was analysed with the aim of differentiating the strains. Pulsed field electrophoresis (TAFE) was used to separate large DNA fragments created by the restriction enzymes NotI, SfiI and ApaI, which specifi...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1993-07, Vol.39 (4-5), p.547-552
Main Authors: LAMOUREUX, M, PREVOST, H, CAVIN, J. F, DIVIES, C
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques
Base Sequence
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
DNA Restriction Enzymes
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Electrophoresis, Gel, Pulsed-Field
Fundamental and applied biological sciences. Psychology
Genetics
Leuconostoc - classification
Leuconostoc - genetics
Leuconostoc oenos
Mission oriented research
RNA Probes
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
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Summary:The chromosome of 41 Leuconostoc oenos strains obtained from collections in different countries was analysed with the aim of differentiating the strains. Pulsed field electrophoresis (TAFE) was used to separate large DNA fragments created by the restriction enzymes NotI, SfiI and ApaI, which specifically recognize guanines or cytosines. The genomic DNA of 11 strains was analysed initially with NotI and only four different restriction profiles were observed. The genome size ranged from 1.8 to 2.1 megabase pairs (Mbp). Constant field electrophoresis applied to DNA treatment with 19 different restriction enzymes showed that the size of the fragments obtained increased proportionally to the percentage G + C present at the site of restriction. EcoRI and HindIII profiles revealed that the zone between 9 and 23 kbp allowed differentiation of the strains tested. Thus, the 41 strains fell into 30 restriction groups using only two enzymes. Hybridization with a non-radioactive DNA probe coding for 16S rRNA revealed that there were two 16S genes on the chromosome.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00205049