BUD2 encodes a GTPase-activating protein for Bud1/Rsr1 necessary for proper bud-site selection in yeast

Cells of the budding yeast Saccharomyces cerevisiae bud at either axial or bipolar sites depending on their cell type. Bud-site selection directs both cell polarity and the cytoskeletal orientation during budding and is determined by at least five genes: BUD1/RSR1, BUD2, BUD3, BUD4 and BUD5. Mutants...

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Bibliographic Details
Published in:Nature (London) 1993-09, Vol.365 (6443), p.269-274
Main Authors: Park, H.O, Chant, J, Herskowitz, I
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Base Sequence
Biochemistry
Biological and medical sciences
cloning
Cloning, Molecular
DNA, Fungal
Enzyme Activation
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
Fungal Proteins - metabolism
genbank/l19162
genes
Genes, Fungal
Genetics
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
GTP Phosphohydrolase Activators
GTP Phosphohydrolases - metabolism
GTPase-Activating Proteins
Humans
Hydrolysis
Microbiology
Molecular Sequence Data
mutants
Mutation
Mycology
nucleotide sequences
Proteins
Restriction Mapping
Saccharomyces
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
Site selection
Yeast
Yeasts
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Description
Summary:Cells of the budding yeast Saccharomyces cerevisiae bud at either axial or bipolar sites depending on their cell type. Bud-site selection directs both cell polarity and the cytoskeletal orientation during budding and is determined by at least five genes: BUD1/RSR1, BUD2, BUD3, BUD4 and BUD5. Mutants defective in BUD1, BUD2 or BUD5 choose bud sites randomly. Bud1 protein (Bud1p) has sequence similarity to Ras, a small GTP-binding protein, and Bud5p is similar to Cdc25p (refs 4, 5), a GDP-GTP exchange factor. Here we report that Bud2p is a GTPase activating protein (GAP) for Bud1p with a sequence similar to the catalytic domain of rasGAPs, and that Bud2p purified from yeast stimulates GTP hydrolysis by Bud1p. Chromosomal deletion of BUD2 causes a random budding pattern but no obvious growth defect. Overexpression of BUD2 also causes a random budding pattern by wild-type cells.
ISSN:0028-0836
1476-4687
DOI:10.1038/365269a0