Simulation of the PCR amplification as two-type-particle branching process

Currently, polymerase chain reaction (PCR) is the main molecular genetic method used for qualitative and quantitative analysis of nucleic acid specific sequences. PCR is based on the branched chain reaction of replication of a double stranded DNA fragment (fragment replication). The reaction is carr...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2010-10, Vol.434 (1), p.239-241
Main Authors: Sochivko, D. G., Fedorov, A. A., Varlamov, D. A., Kurochkin, V. E., Petrov, R. V.
Format: Article
Language:English
Subjects:
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biophysics and Molecular Biology
Life Sciences
Models, Theoretical
Molecular biology
Polymerase chain reaction
Polymerase Chain Reaction - methods
Probability
Simulation
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Summary:Currently, polymerase chain reaction (PCR) is the main molecular genetic method used for qualitative and quantitative analysis of nucleic acid specific sequences. PCR is based on the branched chain reaction of replication of a double stranded DNA fragment (fragment replication). The reaction is carried out by cyclically repeating two stages: (1) denaturation, when complementary strands of the amplified double stranded fragment are separated from one another upon the reaction mixture heating and (2) elongation, during which short specific DNA strands (primers), which are present in the reaction mixture in excess, bind to single strands of the fragment upon the reaction mixture chilling and the complementary DNA strand is synthesized on the single stranded template. The result of these two stages is the doubling of the initial amplified fragment.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672910050054